Two nucleotides 5'-adjacent to the anticodon of rat cytoplasmic tRNA(Asp) are not edited

K. Tomita, T. Ueda, K. Watanabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytoplasmic tRNA(Asp) of rat liver was purified by the solid-phase hybridization method and its nucleotide sequence was analyzed by Donis-Keller's method. The results suggested that the two nucleotides next to the anticodon were identical to its gene sequence, a finding that is inconsistent with a previous report demonstrating by several methods that C32 and T33 on the tRNA(Asp) gene are post-transcriptionally converted to U32 and C33, respectively. Our results indicate that the tRNA hybridized to an oligonucleotide, designed on the basis of the tRNA(Asp) gene sequence, undergoes no editing and possesses C32 and U33 as predicted from the DNA sequence. Analysis of cDNA synthesized from the purified tRNA(Asp) by the RT-PCR method supported the finding that RNA editing is not involved in the maturation process of rat cytoplasmic tRNA(Asp).

Original languageEnglish
Pages (from-to)1001-1006
Number of pages6
JournalBiochimie
Volume78
Issue number11-12
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Keywords

  • Anticodon loop
  • Post-transcriptional modification
  • Queuosine
  • RNA editing
  • tRNA

ASJC Scopus subject areas

  • Biochemistry

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