Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

Kyoichi Azuma, Masaki Kobayashi, Masahisa Nakamura, Nobuo Suzuki, Sayaka Yashima, Shawichi Iwamuro, Mika Ikegame, Toshio Yamamoto, Atsuhiko Hattori

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale.

    Original languageEnglish
    Pages (from-to)594-600
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume362
    Issue number3
    DOIs
    Publication statusPublished - 2007 Oct 26

    Fingerprint

    Cathepsin K
    Goldfish
    Osteoclasts
    Acid Phosphatase
    Messenger RNA
    Mitochondria
    Mammals
    Lysosomes
    Fish
    Fishes
    Complementary DNA
    Calcium
    Molecules
    Tartrate-Resistant Acid Phosphatase
    tartaric acid

    Keywords

    • Bone
    • Cathepsin K
    • Goldfish
    • Morphology
    • Osteoclasts
    • Scale
    • Sequence
    • Tartrate-resistant acid phosphatase

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Azuma, K., Kobayashi, M., Nakamura, M., Suzuki, N., Yashima, S., Iwamuro, S., ... Hattori, A. (2007). Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales. Biochemical and Biophysical Research Communications, 362(3), 594-600. https://doi.org/10.1016/j.bbrc.2007.08.010

    Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales. / Azuma, Kyoichi; Kobayashi, Masaki; Nakamura, Masahisa; Suzuki, Nobuo; Yashima, Sayaka; Iwamuro, Shawichi; Ikegame, Mika; Yamamoto, Toshio; Hattori, Atsuhiko.

    In: Biochemical and Biophysical Research Communications, Vol. 362, No. 3, 26.10.2007, p. 594-600.

    Research output: Contribution to journalArticle

    Azuma, K, Kobayashi, M, Nakamura, M, Suzuki, N, Yashima, S, Iwamuro, S, Ikegame, M, Yamamoto, T & Hattori, A 2007, 'Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales', Biochemical and Biophysical Research Communications, vol. 362, no. 3, pp. 594-600. https://doi.org/10.1016/j.bbrc.2007.08.010
    Azuma, Kyoichi ; Kobayashi, Masaki ; Nakamura, Masahisa ; Suzuki, Nobuo ; Yashima, Sayaka ; Iwamuro, Shawichi ; Ikegame, Mika ; Yamamoto, Toshio ; Hattori, Atsuhiko. / Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 362, No. 3. pp. 594-600.
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