Two-peaked synchronization in day/night expression rhythms of the fibrinogen gene cluster in the mouse liver

Eiko Sakao, Akinori Ishihara, Kazumasa Horikawa, Masashi Akiyama, Makoto Arai, Masaki Kato, Naohiko Seki, Kohji Fukunaga, Atsuko Shimizu-Yabe, Katsuro Iwase, Satoko Ohtsuka, Takeyuki Sato, Yoichi Kohno, Shigenobu Shibata, Masaki Takiguchi

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    21 Citations (Scopus)

    Abstract

    Genes expressed with day/night rhythms in the mouse liver were searched for by microarray analysis using an in-house array harboring mouse liver cDNAs. The rhythmic expression with a single peak and trough level was confirmed by RNA blot analysis for 3β-Hsd and Gabarapll genes exhibiting a peak in the light phase and Spot14, Hspa8, Hspa5, and Hsp84-1 genes showing a peak in the dark phase. On the other hand, mRNA levels for all of the three fibrinogen subunits, Aα, Bβ and γ, exhibited two peaks each in the light and dark phases in a synchronized manner. This two-peaked rhythmic pattern of fibrinogen genes as well as the single peak-trough pattern of other genes was diminished or almost completely lost in the liver of Clock mutant mice, suggesting that the two-peaked expression is also under the control of oscillation-generating genes. In constant darkness, the first peak of the expression rhythm of fibrinogen genes was almost intact, but the second peak disappeared. Therefore, although the first peak in the subjective day is a component of the innate circadian rhythm, the second peak seems to require light stimuli. Fasting in constant darkness caused shifts of time phases of the circadian rhythms. Protein levels of the fibrinogen subunits in whole blood also exhibited circadian rhythms. In the mouse and human loci of the fibrinogen gene cluster, a number of sequence elements resembling circadian transcription factor-binding sites were found. The fibrinogen gene locus provides a unique system for the study of two-peaked day/night rhythms of gene expression in a synchronized form.

    Original languageEnglish
    Pages (from-to)30450-30457
    Number of pages8
    JournalJournal of Biological Chemistry
    Volume278
    Issue number33
    DOIs
    Publication statusPublished - 2003 Aug 15

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    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Sakao, E., Ishihara, A., Horikawa, K., Akiyama, M., Arai, M., Kato, M., Seki, N., Fukunaga, K., Shimizu-Yabe, A., Iwase, K., Ohtsuka, S., Sato, T., Kohno, Y., Shibata, S., & Takiguchi, M. (2003). Two-peaked synchronization in day/night expression rhythms of the fibrinogen gene cluster in the mouse liver. Journal of Biological Chemistry, 278(33), 30450-30457. https://doi.org/10.1074/jbc.M304809200