Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

Y. Hirowatari*, M. Hijikata, Y. Tanji, H. Nyunoya, H. Mizushima, K. Kimura, T. Tanaka, N. Kato, K. Shimotohno

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalArchives of Virology
Volume133
Issue number3-4
DOIs
Publication statusPublished - 1993 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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