Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

Y. Hirowatari, M. Hijikata, Y. Tanji, Hiroshi Nyunoya, H. Mizushima, K. Kimura, T. Tanaka, N. Kato, K. Shimotohno

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalArchives of Virology
Volume133
Issue number3-4
DOIs
Publication statusPublished - 1993 Sep
Externally publishedYes

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Baculoviridae
Insects
Peptide Hydrolases
Peptides
Viral Proteins
Amino Acid Sequence
Amino Acids

ASJC Scopus subject areas

  • Virology

Cite this

Hirowatari, Y., Hijikata, M., Tanji, Y., Nyunoya, H., Mizushima, H., Kimura, K., ... Shimotohno, K. (1993). Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector. Archives of Virology, 133(3-4), 349-356. https://doi.org/10.1007/BF01313774

Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector. / Hirowatari, Y.; Hijikata, M.; Tanji, Y.; Nyunoya, Hiroshi; Mizushima, H.; Kimura, K.; Tanaka, T.; Kato, N.; Shimotohno, K.

In: Archives of Virology, Vol. 133, No. 3-4, 09.1993, p. 349-356.

Research output: Contribution to journalArticle

Hirowatari, Y, Hijikata, M, Tanji, Y, Nyunoya, H, Mizushima, H, Kimura, K, Tanaka, T, Kato, N & Shimotohno, K 1993, 'Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector', Archives of Virology, vol. 133, no. 3-4, pp. 349-356. https://doi.org/10.1007/BF01313774
Hirowatari, Y. ; Hijikata, M. ; Tanji, Y. ; Nyunoya, Hiroshi ; Mizushima, H. ; Kimura, K. ; Tanaka, T. ; Kato, N. ; Shimotohno, K. / Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector. In: Archives of Virology. 1993 ; Vol. 133, No. 3-4. pp. 349-356.
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