Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo

Kyosuke Shinohara, Aiko Kawasumi, Atsuko Takamatsu, Satoko Yoshiba, Yanick Botilde, Noboru Motoyama, Walter Reith, Bénédicte Durand, Hidetaka Shiratori, Hiroshi Hamada

    Research output: Contribution to journalArticle

    77 Citations (Scopus)

    Abstract

    Determination of left-right asymmetry in mouse embryos is achieved by a leftward fluid flow (nodal flow) in the node cavity that is generated by clockwise rotational movement of 200-300 cilia in the node. The precise action of nodal flow and how much flow input is required for the robust read-out of left-right determination remains unknown. Here we show that a local leftward flow generated by as few as two rotating cilia is sufficient to break left-right symmetry. Quantitative analysis of fluid flow and ciliary rotation in the node of mouse embryos shows that left-right asymmetry is already established within a few hours after the onset of rotation by a subset of nodal cilia. Examination of various ciliary mutant mice shows that two rotating cilia are sufficient to initiate left-right asymmetric gene expression. Our results suggest the existence of a highly sensitive system in the node that is able to sense an extremely weak unidirectional flow, and may favour a model in which the flow is sensed as a mechanical force.

    Original languageEnglish
    Article number622
    JournalNature Communications
    Volume3
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Cilia
    embryos
    mice
    Flow of fluids
    Embryonic Structures
    cavities
    symmetry
    Gene expression
    fluid flow
    Chemical analysis
    asymmetry
    gene expression
    Gene Expression
    quantitative analysis
    set theory
    examination

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Chemistry(all)
    • Physics and Astronomy(all)

    Cite this

    Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo. / Shinohara, Kyosuke; Kawasumi, Aiko; Takamatsu, Atsuko; Yoshiba, Satoko; Botilde, Yanick; Motoyama, Noboru; Reith, Walter; Durand, Bénédicte; Shiratori, Hidetaka; Hamada, Hiroshi.

    In: Nature Communications, Vol. 3, 622, 2012.

    Research output: Contribution to journalArticle

    Shinohara, K, Kawasumi, A, Takamatsu, A, Yoshiba, S, Botilde, Y, Motoyama, N, Reith, W, Durand, B, Shiratori, H & Hamada, H 2012, 'Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo', Nature Communications, vol. 3, 622. https://doi.org/10.1038/ncomms1624
    Shinohara, Kyosuke ; Kawasumi, Aiko ; Takamatsu, Atsuko ; Yoshiba, Satoko ; Botilde, Yanick ; Motoyama, Noboru ; Reith, Walter ; Durand, Bénédicte ; Shiratori, Hidetaka ; Hamada, Hiroshi. / Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo. In: Nature Communications. 2012 ; Vol. 3.
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    AU - Yoshiba, Satoko

    AU - Botilde, Yanick

    AU - Motoyama, Noboru

    AU - Reith, Walter

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