Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo

Kyosuke Shinohara, Aiko Kawasumi, Atsuko Takamatsu, Satoko Yoshiba, Yanick Botilde, Noboru Motoyama, Walter Reith, Bénédicte Durand, Hidetaka Shiratori, Hiroshi Hamada

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89 Citations (Scopus)

Abstract

Determination of left-right asymmetry in mouse embryos is achieved by a leftward fluid flow (nodal flow) in the node cavity that is generated by clockwise rotational movement of 200-300 cilia in the node. The precise action of nodal flow and how much flow input is required for the robust read-out of left-right determination remains unknown. Here we show that a local leftward flow generated by as few as two rotating cilia is sufficient to break left-right symmetry. Quantitative analysis of fluid flow and ciliary rotation in the node of mouse embryos shows that left-right asymmetry is already established within a few hours after the onset of rotation by a subset of nodal cilia. Examination of various ciliary mutant mice shows that two rotating cilia are sufficient to initiate left-right asymmetric gene expression. Our results suggest the existence of a highly sensitive system in the node that is able to sense an extremely weak unidirectional flow, and may favour a model in which the flow is sensed as a mechanical force.

Original languageEnglish
Article number622
JournalNature communications
Volume3
DOIs
Publication statusPublished - 2012 Feb 13

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Shinohara, K., Kawasumi, A., Takamatsu, A., Yoshiba, S., Botilde, Y., Motoyama, N., Reith, W., Durand, B., Shiratori, H., & Hamada, H. (2012). Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo. Nature communications, 3, [622]. https://doi.org/10.1038/ncomms1624