Two Sox9 messenger RNA isoforms: Isolation of cDNAs and their expression during gonadal development in the frog Rana rugosa

Minoru Takase, Satoru Noguchi, Masahisa Nakamura

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Sox is a family of SRY-related testis-determining genes. We have isolated two different mRNA isoforms of the frog Sox9 gene from adult frog testis cDNAs. One form (Sox9 α) encodes a 482 amino acid protein containing the HMG box, whereas the other form (Sox9 β), which completely lacks the HMG box, is a truncated 265 amino acid protein of Sox9 α. Sox9 α is 82% similar to mouse, 86% to chicken, and 77% to trout Sox9 at the amino acid level. Sox9 expression was up-regulated in embryos after stage 16, and was seen in both developing testes and ovaries. The size of Sox9 transcripts was determined to be 7.8 knt by Northern blot analysis. In addition, Sox9 α expression was found prominently in the testis and brain among various tissues of adult frogs examined, and was considerably higher than Sox9 β. The fact that Sox9 is expressed in both sexes suggests that this gene is involved in gonadal development of male and female frogs. This is dissimilar to the pattern in birds and mammals, in which Sox9 expression is male-specific. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalFEBS Letters
Volume466
Issue number2-3
DOIs
Publication statusPublished - 2000 Jan 28
Externally publishedYes

Fingerprint

RNA Isoforms
Ranidae
Anura
Testis
Complementary DNA
Genes
Amino Acids
HMGB Proteins
Mammals
Birds
Trout
Brain
Proteins
Northern Blotting
Tissue
Ovary
Chickens
Embryonic Structures

Keywords

  • cDNA cloning
  • Expression in the gonad
  • Frog
  • Isoform
  • Sox9

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Two Sox9 messenger RNA isoforms : Isolation of cDNAs and their expression during gonadal development in the frog Rana rugosa. / Takase, Minoru; Noguchi, Satoru; Nakamura, Masahisa.

In: FEBS Letters, Vol. 466, No. 2-3, 28.01.2000, p. 249-254.

Research output: Contribution to journalArticle

Takase, Minoru ; Noguchi, Satoru ; Nakamura, Masahisa. / Two Sox9 messenger RNA isoforms : Isolation of cDNAs and their expression during gonadal development in the frog Rana rugosa. In: FEBS Letters. 2000 ; Vol. 466, No. 2-3. pp. 249-254.
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