Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes: Molecular cloning and nucleotide sequences

Toshiyuki Miyata, Hiroaki Matsumoto, Masahira Hattori, Yoshiyuki Sakaki, Sadaaki Iwanaga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The complete cDNA sequence coding for the coagulogen present in the horseshoe crab (Tachypleus tridentatus) hemocytes was determined. Clones carrying cDNA fragments for coagulogen were isolated from a cDNA library of the hemocyte mRNA using synthetic oligodeoxyribonuclcotides as probes. The nucleotide sequence analyses of the cloned cDNAs revealed that the hemocyte coagulogen consists of 175 amino acids with 20 amino acids in a presegment, and that there are two types of mRNAs for coagulogen. The two mRNAs exhibited three nucleotide substitutions, two of which were in their protein-coding regions, resulting in two amino acid replacements. Subsequently, two molecular species of coagulogen, named coagulogens type I and type II, were identified by tryptic peptide mapping of the mature proteins isolated from the hemocyte lysate. These results suggest that the two types of coagulogens are first synthesized as preproteins and are incorporated into the granules that are abundantly present in the hemocytes with liberation of the signal peptides.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalJournal of Biochemistry
Volume100
Issue number1
Publication statusPublished - 1986 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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