Tying a molecular knot with optical tweezers

Yasuharu Arai, Ryohei Yasuda, Ken Ichirou Akashi, Yoshie Harada, Hidetake Miyata, Kazuhiko Kinosita, Hiroyasu Itoh

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Filamentous structures are abundant in cells. Relatively rigid filaments, such as microtubules and actin, serve as intracellular scaffolds that support movement and force, and their mechanical properties are crucial to their function in the cell. Some aspects of the behaviour of DNA, meanwhile, depend critically on its flexibility - for example, DNA-binding proteins can induce sharp bends in the helix. The mechanical characterization of such filaments has generally been conducted without controlling the filament shape, by the observation of thermal motions or of the response to external forces or flows. Controlled buckling of a microtubule has been reported, but the analysis of the buckled shape was complicated. Here we report the continuous control of the radius of curvature of a molecular strand by tying a knot in it, using optical tweezers to manipulate the strand's ends. We find that actin filaments break at the knot when the knot diameter fails below 0.4 μm. The pulling force at breakage is around 1 pN, two orders of magnitude smaller than the tensile stress of a straight filament. The flexural rigidity of the filament remained unchanged down to this diameter. We have also knotted a single DNA molecule, opening up the possibility of studying curvature-dependent interactions with associated proteins. We find that the knotted DNA is stronger than actin.

Original languageEnglish
Pages (from-to)446-448
Number of pages3
JournalNature
Volume399
Issue number6735
DOIs
Publication statusPublished - 1999 Jun 3
Externally publishedYes

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Optical Tweezers
Microtubules
Actins
DNA
DNA-Binding Proteins
Actin Cytoskeleton
Hot Temperature
Observation
Proteins

ASJC Scopus subject areas

  • General

Cite this

Arai, Y., Yasuda, R., Akashi, K. I., Harada, Y., Miyata, H., Kinosita, K., & Itoh, H. (1999). Tying a molecular knot with optical tweezers. Nature, 399(6735), 446-448. https://doi.org/10.1038/20894

Tying a molecular knot with optical tweezers. / Arai, Yasuharu; Yasuda, Ryohei; Akashi, Ken Ichirou; Harada, Yoshie; Miyata, Hidetake; Kinosita, Kazuhiko; Itoh, Hiroyasu.

In: Nature, Vol. 399, No. 6735, 03.06.1999, p. 446-448.

Research output: Contribution to journalArticle

Arai, Y, Yasuda, R, Akashi, KI, Harada, Y, Miyata, H, Kinosita, K & Itoh, H 1999, 'Tying a molecular knot with optical tweezers', Nature, vol. 399, no. 6735, pp. 446-448. https://doi.org/10.1038/20894
Arai Y, Yasuda R, Akashi KI, Harada Y, Miyata H, Kinosita K et al. Tying a molecular knot with optical tweezers. Nature. 1999 Jun 3;399(6735):446-448. https://doi.org/10.1038/20894
Arai, Yasuharu ; Yasuda, Ryohei ; Akashi, Ken Ichirou ; Harada, Yoshie ; Miyata, Hidetake ; Kinosita, Kazuhiko ; Itoh, Hiroyasu. / Tying a molecular knot with optical tweezers. In: Nature. 1999 ; Vol. 399, No. 6735. pp. 446-448.
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