Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar Purkinje neurons

Takafumi Inoue, Kunio Kato, Kazuhisa Kohda, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.

Original languageEnglish
Pages (from-to)5366-5373
Number of pages8
JournalJournal of Neuroscience
Volume18
Issue number14
Publication statusPublished - 1998 Jul 15
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Purkinje Cells
Neuronal Plasticity
Cerebellum
Cell Membrane
Antibodies
Brain
Genes

Keywords

  • Brain slice
  • Caged-InsP3
  • Cerebellar Purkinje neuron
  • Longterm depression
  • Patch recording
  • Synaptic plasticity
  • Type 1 inositol 1,4,5-trisphosphate receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar Purkinje neurons. / Inoue, Takafumi; Kato, Kunio; Kohda, Kazuhisa; Mikoshiba, Katsuhiko.

In: Journal of Neuroscience, Vol. 18, No. 14, 15.07.1998, p. 5366-5373.

Research output: Contribution to journalArticle

@article{1b772406c70e40b2b4f7e921a5d2b0d3,
title = "Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar Purkinje neurons",
abstract = "The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.",
keywords = "Brain slice, Caged-InsP3, Cerebellar Purkinje neuron, Longterm depression, Patch recording, Synaptic plasticity, Type 1 inositol 1,4,5-trisphosphate receptor",
author = "Takafumi Inoue and Kunio Kato and Kazuhisa Kohda and Katsuhiko Mikoshiba",
year = "1998",
month = "7",
day = "15",
language = "English",
volume = "18",
pages = "5366--5373",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "14",

}

TY - JOUR

T1 - Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar Purkinje neurons

AU - Inoue, Takafumi

AU - Kato, Kunio

AU - Kohda, Kazuhisa

AU - Mikoshiba, Katsuhiko

PY - 1998/7/15

Y1 - 1998/7/15

N2 - The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.

AB - The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.

KW - Brain slice

KW - Caged-InsP3

KW - Cerebellar Purkinje neuron

KW - Longterm depression

KW - Patch recording

KW - Synaptic plasticity

KW - Type 1 inositol 1,4,5-trisphosphate receptor

UR - http://www.scopus.com/inward/record.url?scp=0032528155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032528155&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 5366

EP - 5373

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 14

ER -