Type i interferon imposes a TSG101/ISG15 checkpoint at the Golgi for glycoprotein trafficking during influenza virus infection

Sumana Sanyal, Joseph Ashour, Takeshi Maruyama, Arwen F. Altenburg, Juan Jose Cragnolini, Angelina Bilate, Ana M. Avalos, Lenka Kundrat, Adolfo García-Sastre, Hidde L. Ploegh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Several enveloped viruses exploit host pathways, such as the cellular endosomal sorting complex required for transport (ESCRT) machinery, for their assembly and release. The influenza A virus (IAV) matrix protein binds to the ESCRT-I complex, although the involvement of early ESCRT proteins such as Tsg101 in IAV trafficking remain to be established. We find that Tsg101 can facilitate IAV trafficking, but this is effectively restricted by the interferon (IFN)-stimulated protein ISG15. Cytosol from type I IFN-treated cells abolished IAV hemagglutinin (HA) transport to the cell surface in infected semi-intact cells. This inhibition required Tsg101 and could be relieved with deISGylases. Tsg101 is itself ISGylated in IFN-treated cells. Upon infection, intact Tsg101-deficient cells obtained by CRISPR-Cas9 genome editing were defective in the surface display of HA and for infectious virion release. These data support the IFN-induced generation of a Tsg101- and ISG15-dependent checkpoint in the secretory pathway that compromises influenza virus release.

Original languageEnglish
Pages (from-to)510-521
Number of pages12
JournalCell Host and Microbe
Volume14
Issue number5
DOIs
Publication statusPublished - 2013 Nov 13
Externally publishedYes

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Virus Diseases
Orthomyxoviridae
Endosomal Sorting Complexes Required for Transport
Interferons
Glycoproteins
Influenza A virus
Hemagglutinins
Clustered Regularly Interspaced Short Palindromic Repeats
Virus Release
Interferon Type I
Secretory Pathway
Virion
Cytosol
Carrier Proteins
Viruses
Infection
Proteins

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Type i interferon imposes a TSG101/ISG15 checkpoint at the Golgi for glycoprotein trafficking during influenza virus infection. / Sanyal, Sumana; Ashour, Joseph; Maruyama, Takeshi; Altenburg, Arwen F.; Cragnolini, Juan Jose; Bilate, Angelina; Avalos, Ana M.; Kundrat, Lenka; García-Sastre, Adolfo; Ploegh, Hidde L.

In: Cell Host and Microbe, Vol. 14, No. 5, 13.11.2013, p. 510-521.

Research output: Contribution to journalArticle

Sanyal, S, Ashour, J, Maruyama, T, Altenburg, AF, Cragnolini, JJ, Bilate, A, Avalos, AM, Kundrat, L, García-Sastre, A & Ploegh, HL 2013, 'Type i interferon imposes a TSG101/ISG15 checkpoint at the Golgi for glycoprotein trafficking during influenza virus infection', Cell Host and Microbe, vol. 14, no. 5, pp. 510-521. https://doi.org/10.1016/j.chom.2013.10.011
Sanyal, Sumana ; Ashour, Joseph ; Maruyama, Takeshi ; Altenburg, Arwen F. ; Cragnolini, Juan Jose ; Bilate, Angelina ; Avalos, Ana M. ; Kundrat, Lenka ; García-Sastre, Adolfo ; Ploegh, Hidde L. / Type i interferon imposes a TSG101/ISG15 checkpoint at the Golgi for glycoprotein trafficking during influenza virus infection. In: Cell Host and Microbe. 2013 ; Vol. 14, No. 5. pp. 510-521.
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