Typing of X Chromosomes Bearing Tabby Allele in Mouse Preimplantation Embryos by Detection of a Microsatellite Marker

Ruby P A Valdivia, Tetsuo Kunieda, Masahira Hattori, Yutaka Toyoda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.

Original languageEnglish
Pages (from-to)395-398
Number of pages4
JournalExperimental Animals
Volume45
Issue number4
Publication statusPublished - 1996 Oct
Externally publishedYes

Keywords

  • Microsatellite
  • Tabby
  • XO

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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