Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest

Takayuki Ogawa, Harumi Furochi, Mai Mameoka, Katsuya Hirasaka, Yuko Onishi, Naoto Suzue, Motoko Oarada, Motoki Akamatsu, Hiroshi Akima, Tetsuo Fukunaga, Kyoichi Kishi, Natsuo Yasui, Kazumi Ishidoh, Hideoki Fukuoka, Takeshi Nikawa

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

In animal models, several ubiquitin ligases play an important role in skeletal muscle atrophy caused by unloading. In this study we examined protein ubiquitination and ubiquitin ligase gene expression in quadriceps femoris muscle from healthy volunteers after 20-day bedrest to clarify ubiquitin-dependent proteolysis in human muscles after unloading. During bedrest, thickness and cross-sectional area of the quadriceps femoris muscle decreased significantly by 4.6% and 3.7%, respectively. Ubiquitinated proteins accumulated in these atrophied human muscles. A real-time reverse transcription-polymerase chain reaction system showed that bedrest significantly upregulated expression of two ubiquitin ligase genes, Cbl-b and atrogin-1. We also performed DNA microarray analysis to examine comprehensive gene expression in the atrophied muscle. Bedrest mainly suppressed the expression of muscle genes associated with control of gene expression in skeletal muscle. Our results suggest that, in humans, Cbl-b- or atrogin-1-mediated ubiquitination plays an important role in unloading-induced muscle atrophy, and that unloading stress may preferentially inhibit transcriptional responses in skeletal muscle.

Original languageEnglish
Pages (from-to)463-469
Number of pages7
JournalMuscle and Nerve
Volume34
Issue number4
DOIs
Publication statusPublished - 2006 Oct
Externally publishedYes

Fingerprint

Bed Rest
Quadriceps Muscle
Ligases
Ubiquitin
Healthy Volunteers
Gene Expression
Muscles
Skeletal Muscle
Muscular Atrophy
Ubiquitination
Ubiquitinated Proteins
Ubiquitin-Protein Ligases
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Proteolysis
Reverse Transcription
Animal Models
Polymerase Chain Reaction
Genes

Keywords

  • Bedrest
  • cDNA microarray
  • Skeletal muscle atrophy
  • Ubiquitin-dependent proteolytic pathway

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Ogawa, T., Furochi, H., Mameoka, M., Hirasaka, K., Onishi, Y., Suzue, N., ... Nikawa, T. (2006). Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest. Muscle and Nerve, 34(4), 463-469. https://doi.org/10.1002/mus.20611

Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest. / Ogawa, Takayuki; Furochi, Harumi; Mameoka, Mai; Hirasaka, Katsuya; Onishi, Yuko; Suzue, Naoto; Oarada, Motoko; Akamatsu, Motoki; Akima, Hiroshi; Fukunaga, Tetsuo; Kishi, Kyoichi; Yasui, Natsuo; Ishidoh, Kazumi; Fukuoka, Hideoki; Nikawa, Takeshi.

In: Muscle and Nerve, Vol. 34, No. 4, 10.2006, p. 463-469.

Research output: Contribution to journalArticle

Ogawa, T, Furochi, H, Mameoka, M, Hirasaka, K, Onishi, Y, Suzue, N, Oarada, M, Akamatsu, M, Akima, H, Fukunaga, T, Kishi, K, Yasui, N, Ishidoh, K, Fukuoka, H & Nikawa, T 2006, 'Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest', Muscle and Nerve, vol. 34, no. 4, pp. 463-469. https://doi.org/10.1002/mus.20611
Ogawa T, Furochi H, Mameoka M, Hirasaka K, Onishi Y, Suzue N et al. Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest. Muscle and Nerve. 2006 Oct;34(4):463-469. https://doi.org/10.1002/mus.20611
Ogawa, Takayuki ; Furochi, Harumi ; Mameoka, Mai ; Hirasaka, Katsuya ; Onishi, Yuko ; Suzue, Naoto ; Oarada, Motoko ; Akamatsu, Motoki ; Akima, Hiroshi ; Fukunaga, Tetsuo ; Kishi, Kyoichi ; Yasui, Natsuo ; Ishidoh, Kazumi ; Fukuoka, Hideoki ; Nikawa, Takeshi. / Ubiquitin ligase gene expression in healthy volunteers with 20-day bedrest. In: Muscle and Nerve. 2006 ; Vol. 34, No. 4. pp. 463-469.
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