Ultimate hydrogel thermal-transition based flow control system for user-friendly particle and cell sorting

H. Sugino, K. Ozaki, Y. Shirasaki, T. Aoki, T. Arakawa, Donghyun Yoon, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports an ultimate hydrogel-based flow control system for fluorescently-labeled bioparticle sorting. We previously developed a hydrogel-based sorter. But the system requires the mixture of bio-sample and hydrogel to perform flow switching. This might be a concern when sorted samples are analyzed by sensitive analytical techniques. To overcome this issue, we developed a new system which doesn't require the mixing of sample and hydrogel. With the system, we successfully sorted microspheres and bacterial cells without mixing. The system is practical for following sample analysis because there is no need to separate sample from hydrogel after sorting.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages244-246
Number of pages3
Volume1
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen
Duration: 2010 Oct 32010 Oct 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CityGroningen
Period10/10/310/10/7

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Keywords

  • Cell
  • Fluorescence-activated cell sorter
  • Sorting
  • Thermoreversible gelation polymer

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Sugino, H., Ozaki, K., Shirasaki, Y., Aoki, T., Arakawa, T., Yoon, D., Shoji, S., & Funatsu, T. (2010). Ultimate hydrogel thermal-transition based flow control system for user-friendly particle and cell sorting. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 1, pp. 244-246)