Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

H. Maruyama, R. Kariya, S. Nakamura, T. Matsuda, yu Matsuda, T. Niimi, A. Honda, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this paper, we proposed long-lifetime and high-sensitive measurement using fluorescent intensity by compensation of photo-bleaching with the difference compensation for cell measurement. Photo-bleaching of fluorescence is one of the most crucial factors to cause measurement error in physiological measurement. In our approach, difference of fluorescent intensity between imaging time is compensated using single exponential function to make the difference amount uniform in each imaging time. We evaluated the dependencies of measurable time and the sensitivity by fluorescent lifetime and the decay coefficient of fluorescent lifetime using numerical simulation. From these results, the proposed method has much higher sensitivity and longer measurable time against conventional compensation method of photo-bleaching. By using the proposed method, we demonstrate the photosynthesis activity of single Synechocystis sp. PCC 6803 in the micro chamber with gas barrier layer.

Original languageEnglish
Title of host publication2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012
Pages3235-3240
Number of pages6
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes
Event25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012 - Vilamoura, Algarve
Duration: 2012 Oct 72012 Oct 12

Other

Other25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012
CityVilamoura, Algarve
Period12/10/712/10/12

Fingerprint

Photobleaching
Imaging techniques
Photosynthesis
Exponential functions
Measurement errors
Fluorescence
Computer simulation
Gases
Compensation and Redress

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Vision and Pattern Recognition
  • Computer Science Applications

Cite this

Maruyama, H., Kariya, R., Nakamura, S., Matsuda, T., Matsuda, Y., Niimi, T., ... Arai, F. (2012). Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis. In 2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012 (pp. 3235-3240). [6386247] https://doi.org/10.1109/IROS.2012.6386247

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis. / Maruyama, H.; Kariya, R.; Nakamura, S.; Matsuda, T.; Matsuda, yu; Niimi, T.; Honda, A.; Arai, F.

2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012. 2012. p. 3235-3240 6386247.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Maruyama, H, Kariya, R, Nakamura, S, Matsuda, T, Matsuda, Y, Niimi, T, Honda, A & Arai, F 2012, Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis. in 2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012., 6386247, pp. 3235-3240, 25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012, Vilamoura, Algarve, 12/10/7. https://doi.org/10.1109/IROS.2012.6386247
Maruyama H, Kariya R, Nakamura S, Matsuda T, Matsuda Y, Niimi T et al. Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis. In 2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012. 2012. p. 3235-3240. 6386247 https://doi.org/10.1109/IROS.2012.6386247
Maruyama, H. ; Kariya, R. ; Nakamura, S. ; Matsuda, T. ; Matsuda, yu ; Niimi, T. ; Honda, A. ; Arai, F. / Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis. 2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012. 2012. pp. 3235-3240
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