Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Yusuke Yoshioka, Nobuyoshi Kosaka, Yuki Konishi, Hideki Ohta, Hiroyuki Okamoto, Hikaru Sonoda, Ryoji Nonaka, Hirofumi Yamamoto, Hideshi Ishii, Masaki Mori, Koh Furuta, Takeshi Nakajima, Hiroshi Hayashi, Hajime Sugisaki, Hiroko Higashimoto, Takashi Kato, Fumitaka Takeshita, Takahiro Ochiya

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

Original languageEnglish
Pages (from-to)3591
Number of pages1
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014
Externally publishedYes

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CD147 Antigens
Photosensitizing Agents
Biopsy
Tumor Biomarkers
Medicine
Purification
Blood
cancer
Cells
Antibodies
Liquids
liquids
Colorectal Neoplasms
biomarkers
antigens
antibodies
medicine
purification
beads
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yoshioka, Y., Kosaka, N., Konishi, Y., Ohta, H., Okamoto, H., Sonoda, H., ... Ochiya, T. (2014). Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nature Communications, 5, 3591. https://doi.org/10.1038/ncomms4591

Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. / Yoshioka, Yusuke; Kosaka, Nobuyoshi; Konishi, Yuki; Ohta, Hideki; Okamoto, Hiroyuki; Sonoda, Hikaru; Nonaka, Ryoji; Yamamoto, Hirofumi; Ishii, Hideshi; Mori, Masaki; Furuta, Koh; Nakajima, Takeshi; Hayashi, Hiroshi; Sugisaki, Hajime; Higashimoto, Hiroko; Kato, Takashi; Takeshita, Fumitaka; Ochiya, Takahiro.

In: Nature Communications, Vol. 5, 2014, p. 3591.

Research output: Contribution to journalArticle

Yoshioka, Y, Kosaka, N, Konishi, Y, Ohta, H, Okamoto, H, Sonoda, H, Nonaka, R, Yamamoto, H, Ishii, H, Mori, M, Furuta, K, Nakajima, T, Hayashi, H, Sugisaki, H, Higashimoto, H, Kato, T, Takeshita, F & Ochiya, T 2014, 'Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen', Nature Communications, vol. 5, pp. 3591. https://doi.org/10.1038/ncomms4591
Yoshioka, Yusuke ; Kosaka, Nobuyoshi ; Konishi, Yuki ; Ohta, Hideki ; Okamoto, Hiroyuki ; Sonoda, Hikaru ; Nonaka, Ryoji ; Yamamoto, Hirofumi ; Ishii, Hideshi ; Mori, Masaki ; Furuta, Koh ; Nakajima, Takeshi ; Hayashi, Hiroshi ; Sugisaki, Hajime ; Higashimoto, Hiroko ; Kato, Takashi ; Takeshita, Fumitaka ; Ochiya, Takahiro. / Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. In: Nature Communications. 2014 ; Vol. 5. pp. 3591.
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