Ultrasonically-induced electrical potentials in demineralized bovine cortical bone

Shunki Mori, Taiki Makino, Daisuke Koyama, Shinji Takayanagi, Takahiko Yanagitani, Mami Matsukawa

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    While the low-intensity pulsed ultrasound technique has proved useful for healing of bone fractures, the ultrasound healing mechanism is not yet understood. To understand the initial physical effects of the ultrasound irradiation process on bone, we have studied the anisotropic piezoelectric properties of bone in the MHz range. Bone is known to be composed of collagen and hydroxyapatite (HAp) and shows strong elastic anisotropy. In this study, the effects of HAp on the piezoelectricity were investigated experimentally. To remove the HAp crystallites from the bovine cortical bone, demineralization was performed using ethylene diamine tetra-acetic acid (EDTA) solutions. To investigate the piezoelectricity, we have fabricated ultrasound transducers using the cortical bone or demineralized cortical bone. The induced electrical potentials due to the piezoelectricity were observed as the output of these transducers under pulsed ultrasound irradiation in the MHz range. The cortical bone transducer (before mineralization) showed anisotropic piezoelectric behavior. When the ultrasound irradiation was applied normal to the transducer surface, the observed induced electrical potentials had minimum values. The potential increased under off-axis ultrasound irradiation with changes in polarization. In the demineralized bone transducer case, however, the anisotropic behavior was not observed in the induced electrical potentials. These results therefore indicate that the HAp crystallites affect the piezoelectric characteristics of bone.

    Original languageEnglish
    Article number045007
    JournalAIP Advances
    Volume8
    Issue number4
    DOIs
    Publication statusPublished - 2018 Apr 1

    Fingerprint

    bones
    transducers
    piezoelectricity
    irradiation
    healing
    bone demineralization
    crystallites
    elastic anisotropy
    ethylenediamine
    collagens
    acetic acid
    output
    polarization

    ASJC Scopus subject areas

    • Physics and Astronomy(all)

    Cite this

    Ultrasonically-induced electrical potentials in demineralized bovine cortical bone. / Mori, Shunki; Makino, Taiki; Koyama, Daisuke; Takayanagi, Shinji; Yanagitani, Takahiko; Matsukawa, Mami.

    In: AIP Advances, Vol. 8, No. 4, 045007, 01.04.2018.

    Research output: Contribution to journalArticle

    Mori, S, Makino, T, Koyama, D, Takayanagi, S, Yanagitani, T & Matsukawa, M 2018, 'Ultrasonically-induced electrical potentials in demineralized bovine cortical bone', AIP Advances, vol. 8, no. 4, 045007. https://doi.org/10.1063/1.5022138
    Mori, Shunki ; Makino, Taiki ; Koyama, Daisuke ; Takayanagi, Shinji ; Yanagitani, Takahiko ; Matsukawa, Mami. / Ultrasonically-induced electrical potentials in demineralized bovine cortical bone. In: AIP Advances. 2018 ; Vol. 8, No. 4.
    @article{b75764f8873c471f8739dc7dfce75070,
    title = "Ultrasonically-induced electrical potentials in demineralized bovine cortical bone",
    abstract = "While the low-intensity pulsed ultrasound technique has proved useful for healing of bone fractures, the ultrasound healing mechanism is not yet understood. To understand the initial physical effects of the ultrasound irradiation process on bone, we have studied the anisotropic piezoelectric properties of bone in the MHz range. Bone is known to be composed of collagen and hydroxyapatite (HAp) and shows strong elastic anisotropy. In this study, the effects of HAp on the piezoelectricity were investigated experimentally. To remove the HAp crystallites from the bovine cortical bone, demineralization was performed using ethylene diamine tetra-acetic acid (EDTA) solutions. To investigate the piezoelectricity, we have fabricated ultrasound transducers using the cortical bone or demineralized cortical bone. The induced electrical potentials due to the piezoelectricity were observed as the output of these transducers under pulsed ultrasound irradiation in the MHz range. The cortical bone transducer (before mineralization) showed anisotropic piezoelectric behavior. When the ultrasound irradiation was applied normal to the transducer surface, the observed induced electrical potentials had minimum values. The potential increased under off-axis ultrasound irradiation with changes in polarization. In the demineralized bone transducer case, however, the anisotropic behavior was not observed in the induced electrical potentials. These results therefore indicate that the HAp crystallites affect the piezoelectric characteristics of bone.",
    author = "Shunki Mori and Taiki Makino and Daisuke Koyama and Shinji Takayanagi and Takahiko Yanagitani and Mami Matsukawa",
    year = "2018",
    month = "4",
    day = "1",
    doi = "10.1063/1.5022138",
    language = "English",
    volume = "8",
    journal = "AIP Advances",
    issn = "2158-3226",
    publisher = "American Institute of Physics Publising LLC",
    number = "4",

    }

    TY - JOUR

    T1 - Ultrasonically-induced electrical potentials in demineralized bovine cortical bone

    AU - Mori, Shunki

    AU - Makino, Taiki

    AU - Koyama, Daisuke

    AU - Takayanagi, Shinji

    AU - Yanagitani, Takahiko

    AU - Matsukawa, Mami

    PY - 2018/4/1

    Y1 - 2018/4/1

    N2 - While the low-intensity pulsed ultrasound technique has proved useful for healing of bone fractures, the ultrasound healing mechanism is not yet understood. To understand the initial physical effects of the ultrasound irradiation process on bone, we have studied the anisotropic piezoelectric properties of bone in the MHz range. Bone is known to be composed of collagen and hydroxyapatite (HAp) and shows strong elastic anisotropy. In this study, the effects of HAp on the piezoelectricity were investigated experimentally. To remove the HAp crystallites from the bovine cortical bone, demineralization was performed using ethylene diamine tetra-acetic acid (EDTA) solutions. To investigate the piezoelectricity, we have fabricated ultrasound transducers using the cortical bone or demineralized cortical bone. The induced electrical potentials due to the piezoelectricity were observed as the output of these transducers under pulsed ultrasound irradiation in the MHz range. The cortical bone transducer (before mineralization) showed anisotropic piezoelectric behavior. When the ultrasound irradiation was applied normal to the transducer surface, the observed induced electrical potentials had minimum values. The potential increased under off-axis ultrasound irradiation with changes in polarization. In the demineralized bone transducer case, however, the anisotropic behavior was not observed in the induced electrical potentials. These results therefore indicate that the HAp crystallites affect the piezoelectric characteristics of bone.

    AB - While the low-intensity pulsed ultrasound technique has proved useful for healing of bone fractures, the ultrasound healing mechanism is not yet understood. To understand the initial physical effects of the ultrasound irradiation process on bone, we have studied the anisotropic piezoelectric properties of bone in the MHz range. Bone is known to be composed of collagen and hydroxyapatite (HAp) and shows strong elastic anisotropy. In this study, the effects of HAp on the piezoelectricity were investigated experimentally. To remove the HAp crystallites from the bovine cortical bone, demineralization was performed using ethylene diamine tetra-acetic acid (EDTA) solutions. To investigate the piezoelectricity, we have fabricated ultrasound transducers using the cortical bone or demineralized cortical bone. The induced electrical potentials due to the piezoelectricity were observed as the output of these transducers under pulsed ultrasound irradiation in the MHz range. The cortical bone transducer (before mineralization) showed anisotropic piezoelectric behavior. When the ultrasound irradiation was applied normal to the transducer surface, the observed induced electrical potentials had minimum values. The potential increased under off-axis ultrasound irradiation with changes in polarization. In the demineralized bone transducer case, however, the anisotropic behavior was not observed in the induced electrical potentials. These results therefore indicate that the HAp crystallites affect the piezoelectric characteristics of bone.

    UR - http://www.scopus.com/inward/record.url?scp=85045042932&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85045042932&partnerID=8YFLogxK

    U2 - 10.1063/1.5022138

    DO - 10.1063/1.5022138

    M3 - Article

    VL - 8

    JO - AIP Advances

    JF - AIP Advances

    SN - 2158-3226

    IS - 4

    M1 - 045007

    ER -