Unbinding force of a single motor molecule of muscle measured using optical tweezers

Takayuki Nishizaka, Hidetake Miyata, Hiroshi Yoshikawa, Shin'ichi Ishiwata, Kazuhiko Kinosita

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

THE unbinding and rebinding of motor proteins and their substrate filaments are the main components of sliding movement1. We have measured the unbinding force between an actin filament and a single motor molecule of muscle, myosin, in the absence of ATP, by pulling the filament with optical tweezers2. The unbinding force could be measured repeatedly on the same molecule, and was independent of the number of measurements and the direction of the imposed loads within a range of ±90°. The average unbinding force was 9.2 ± 4.4 pN, only a few times larger than the sliding force3"5 but an order of magnitude smaller than other intermolecular forces6,7. From its kinetics8 we suggest that unbinding occurs sequentially at the molecular interface, which is an inherent property of motor molecules.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalNature
Volume377
Issue number6546
Publication statusPublished - 1995
Externally publishedYes

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Optical Tweezers
Myosins
Actin Cytoskeleton
Adenosine Triphosphate
Muscles
Proteins
Direction compound

ASJC Scopus subject areas

  • General

Cite this

Nishizaka, T., Miyata, H., Yoshikawa, H., Ishiwata, S., & Kinosita, K. (1995). Unbinding force of a single motor molecule of muscle measured using optical tweezers. Nature, 377(6546), 251-254.

Unbinding force of a single motor molecule of muscle measured using optical tweezers. / Nishizaka, Takayuki; Miyata, Hidetake; Yoshikawa, Hiroshi; Ishiwata, Shin'ichi; Kinosita, Kazuhiko.

In: Nature, Vol. 377, No. 6546, 1995, p. 251-254.

Research output: Contribution to journalArticle

Nishizaka, T, Miyata, H, Yoshikawa, H, Ishiwata, S & Kinosita, K 1995, 'Unbinding force of a single motor molecule of muscle measured using optical tweezers', Nature, vol. 377, no. 6546, pp. 251-254.
Nishizaka T, Miyata H, Yoshikawa H, Ishiwata S, Kinosita K. Unbinding force of a single motor molecule of muscle measured using optical tweezers. Nature. 1995;377(6546):251-254.
Nishizaka, Takayuki ; Miyata, Hidetake ; Yoshikawa, Hiroshi ; Ishiwata, Shin'ichi ; Kinosita, Kazuhiko. / Unbinding force of a single motor molecule of muscle measured using optical tweezers. In: Nature. 1995 ; Vol. 377, No. 6546. pp. 251-254.
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