UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro

Yoko Ozaki, Toshiharu Suzuki, Yutetsu Kuruma, Takuya Ueda, Masasuke Yoshida

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In FoF1-ATP synthase, multimeric c-subunits are assembled to a ring (c-ring) in the membranes that rotates as protons flow across Fo. We recently reported that assembly of c-ring of Propionigenium modestum in the membranes of Escherichia coli cells required P. modestum UncI, a product of the conserved uncI gene in the FoF1 operon. However, cooperation with endogenous factors in E. coli remained unclear. Here, P. modestum c-subunit was synthesized in vitro in the presence of liposomes. When c-subunit alone was synthesized, it did not form c-ring. However, when c-subunit and P. modestum UncI were synthesized together, c-ring was formed. Fusion of the two kinds of liposomes, one containing only unassembled c-subunit and the other only UncI, resulted in gradual formation of c-ring. Thus, UncI alone can mediate in vitro post-translational c-ring assembly.

Original languageEnglish
Pages (from-to)663-666
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number3
DOIs
Publication statusPublished - 2008 Mar 14
Externally publishedYes

Fingerprint

Liposomes
Propionigenium
Escherichia coli
Adenosine Triphosphate
Membranes
Operon
Protons
Proteins
Fusion reactions
Genes
In Vitro Techniques

Keywords

  • ATP synthase
  • Biogenesis of ATP synthase
  • c-Subunit
  • Chaperone
  • In vitro protein synthesis
  • Membrane integration
  • Membrane proteins
  • Motor enzyme
  • UncI
  • YidC

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro. / Ozaki, Yoko; Suzuki, Toshiharu; Kuruma, Yutetsu; Ueda, Takuya; Yoshida, Masasuke.

In: Biochemical and Biophysical Research Communications, Vol. 367, No. 3, 14.03.2008, p. 663-666.

Research output: Contribution to journalArticle

Ozaki, Yoko ; Suzuki, Toshiharu ; Kuruma, Yutetsu ; Ueda, Takuya ; Yoshida, Masasuke. / UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 367, No. 3. pp. 663-666.
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