Uniform and high throughput agarose gel micro droplet generation device for single cell analysis

T. Hirose, Y. Hoshino, Donghyun Yoon, A. Nakahara, T. Mori, Tetsushi Sekiguchi, Haruko Takeyama, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We designed and fabricated high throughput agarose gel micro droplet (GMD) generation device for single cell function analysis. Temperature control of each elements of the device is important issue because of the thermo-oriented sol-gel transfer of agarose gel. Uniform GMD of 50 μm in diameter which is suitable size for cell culturing media is achieved with size deviation less than 1.58% and generation rate of about 190deoplets/sec. Single E.coli cell encapsulation and cultivation for 5 hours are successfully achieved in an agarose GMD.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages500-502
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Sepharose
Gels
Throughput
Encapsulation
Temperature control
Escherichia coli
Sol-gels

Keywords

  • Agarose gel
  • E.coli
  • Gel micro droplet (GMD)
  • Microfluidic

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Hirose, T., Hoshino, Y., Yoon, D., Nakahara, A., Mori, T., Sekiguchi, T., ... Shoji, S. (2012). Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 500-502). Chemical and Biological Microsystems Society.

Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. / Hirose, T.; Hoshino, Y.; Yoon, Donghyun; Nakahara, A.; Mori, T.; Sekiguchi, Tetsushi; Takeyama, Haruko; Shoji, Shuichi.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 500-502.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hirose, T, Hoshino, Y, Yoon, D, Nakahara, A, Mori, T, Sekiguchi, T, Takeyama, H & Shoji, S 2012, Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 500-502, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 12/10/28.
Hirose T, Hoshino Y, Yoon D, Nakahara A, Mori T, Sekiguchi T et al. Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 500-502
Hirose, T. ; Hoshino, Y. ; Yoon, Donghyun ; Nakahara, A. ; Mori, T. ; Sekiguchi, Tetsushi ; Takeyama, Haruko ; Shoji, Shuichi. / Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 500-502
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