Unique structure of new serine tRNAs responsible for decoding leucine codon CUG in various Candida species and their putative ancestral tRNA genes

Takuya Ueda, T. Suzuki, T. Tokogawa, K. Nishikawa, K. Watanabe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In an asporogenic yeast, Candida cylindracea, codon CUG is not translated as leucine but as serine. On the basis of our recent work on the determination of the genetic code using in vitro translation systems coupled with isolation of the corresponding tRNA molecules, it appears that this non-universal genetic code is unitized not only in C cylindracea but also in various Hemiascomycetes. Here we show that in addition to the species already reported, three pathogenic yeast, C guilliermondii, C lusitaniae and C tropicalis, have tRNASerCAG, indicating that this non-universal genetic code (CUG=Ser) also exists in these species. Determination of their primary structures revealed that the uridine conserved at position 33 in usual tRNAs, is replaced by guanosine or cytidine. This suggests that the three-dimensional structures of the anticodon loop of these tRNAs differ from the conventional structure comprising the U turn in this position. Moreover, we succeeded in isolating putative ancestral serine tRNA genesw hose sequences are highly homologous to tRNASerCAG in each case. These tRNA genes all have the anticodon sequence CGA corresponding to the codon UCG, indicating that tRNASerCAG might have emerged from tRNASerCGA during evolutionary change of the assignment of codon CUG.

Original languageEnglish
Pages (from-to)1217-1222
Number of pages6
JournalBiochimie
Volume76
Issue number12
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Fingerprint

Candida
Transfer RNA
Codon
Leucine
Serine
Decoding
Genetic Code
Genes
Anticodon
Yeast
Yeasts
Cytidine
Hose
Guanosine
Uridine
Sequence Homology
Molecules

Keywords

  • anticodon
  • Candida
  • gene structure
  • non-universal genetic code
  • tRNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Unique structure of new serine tRNAs responsible for decoding leucine codon CUG in various Candida species and their putative ancestral tRNA genes. / Ueda, Takuya; Suzuki, T.; Tokogawa, T.; Nishikawa, K.; Watanabe, K.

In: Biochimie, Vol. 76, No. 12, 01.01.1994, p. 1217-1222.

Research output: Contribution to journalArticle

Ueda, Takuya ; Suzuki, T. ; Tokogawa, T. ; Nishikawa, K. ; Watanabe, K. / Unique structure of new serine tRNAs responsible for decoding leucine codon CUG in various Candida species and their putative ancestral tRNA genes. In: Biochimie. 1994 ; Vol. 76, No. 12. pp. 1217-1222.
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