Unique transcriptional profile of native persisters in Escherichia coli

Shinya Matsumoto, Yuto Kawai, Satoshi Miyagawa, Yuka Iwamoto, Shujiro Okuda, Alicia Sánchez-Gorostiaga, Miguel Vicente, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Non-dividing persisters, bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, are among the many causes of the refractory nature of bacterial infections. Here we constructed a recombinant Escherichia coli strain that enables to distinguish non-dividing from dividing cell based on Z-ring during cell division. Then, non-dividing cells and dividing cells were successfully separated using a fluorescence activated cell sorter. The sorted non-dividing cells showed significantly higher tolerance toward ofloxacin than dividing cells, which indicates that persisters were concentrated with the methodology. Transcriptional analysis revealed that genes involved in guanosine tetraphosphate synthesis are upregulated in persisters, which represses transcription and DNA replication and leads to ofloxacin tolerance. Lactate dehydrogenase and several ATP-binding cassette transporters were upregulated in persisters to adapt to anaerobic metabolism. In addition, nitrite and dimethyl sulfoxide (DMSO) may be used as reducible substrates for alternative energy generation pathways. Our methodology revealed a unique transcriptional profile of E. coli persisters.

    Original languageEnglish
    JournalJournal of Bioscience and Bioengineering
    DOIs
    Publication statusAccepted/In press - 2017

    Fingerprint

    Ofloxacin
    Escherichia coli
    Guanosine Tetraphosphate
    ATP-Binding Cassette Transporters
    Dimethyl sulfoxide
    Adenosinetriphosphate
    Antibiotics
    Transcription
    Nitrites
    Dimethyl Sulfoxide
    L-Lactate Dehydrogenase
    Metabolism
    Refractory materials
    Bacteria
    DNA
    Genes
    Fluorescence
    Cells
    Anti-Bacterial Agents
    Substrates

    Keywords

    • Anaerobic respiration
    • Antibiotics tolerance
    • Cell division
    • Dormant cell
    • Persister

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Unique transcriptional profile of native persisters in Escherichia coli. / Matsumoto, Shinya; Kawai, Yuto; Miyagawa, Satoshi; Iwamoto, Yuka; Okuda, Shujiro; Sánchez-Gorostiaga, Alicia; Vicente, Miguel; Tsuneda, Satoshi.

    In: Journal of Bioscience and Bioengineering, 2017.

    Research output: Contribution to journalArticle

    Matsumoto, Shinya ; Kawai, Yuto ; Miyagawa, Satoshi ; Iwamoto, Yuka ; Okuda, Shujiro ; Sánchez-Gorostiaga, Alicia ; Vicente, Miguel ; Tsuneda, Satoshi. / Unique transcriptional profile of native persisters in Escherichia coli. In: Journal of Bioscience and Bioengineering. 2017.
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    AU - Kawai, Yuto

    AU - Miyagawa, Satoshi

    AU - Iwamoto, Yuka

    AU - Okuda, Shujiro

    AU - Sánchez-Gorostiaga, Alicia

    AU - Vicente, Miguel

    AU - Tsuneda, Satoshi

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    N2 - Non-dividing persisters, bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, are among the many causes of the refractory nature of bacterial infections. Here we constructed a recombinant Escherichia coli strain that enables to distinguish non-dividing from dividing cell based on Z-ring during cell division. Then, non-dividing cells and dividing cells were successfully separated using a fluorescence activated cell sorter. The sorted non-dividing cells showed significantly higher tolerance toward ofloxacin than dividing cells, which indicates that persisters were concentrated with the methodology. Transcriptional analysis revealed that genes involved in guanosine tetraphosphate synthesis are upregulated in persisters, which represses transcription and DNA replication and leads to ofloxacin tolerance. Lactate dehydrogenase and several ATP-binding cassette transporters were upregulated in persisters to adapt to anaerobic metabolism. In addition, nitrite and dimethyl sulfoxide (DMSO) may be used as reducible substrates for alternative energy generation pathways. Our methodology revealed a unique transcriptional profile of E. coli persisters.

    AB - Non-dividing persisters, bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, are among the many causes of the refractory nature of bacterial infections. Here we constructed a recombinant Escherichia coli strain that enables to distinguish non-dividing from dividing cell based on Z-ring during cell division. Then, non-dividing cells and dividing cells were successfully separated using a fluorescence activated cell sorter. The sorted non-dividing cells showed significantly higher tolerance toward ofloxacin than dividing cells, which indicates that persisters were concentrated with the methodology. Transcriptional analysis revealed that genes involved in guanosine tetraphosphate synthesis are upregulated in persisters, which represses transcription and DNA replication and leads to ofloxacin tolerance. Lactate dehydrogenase and several ATP-binding cassette transporters were upregulated in persisters to adapt to anaerobic metabolism. In addition, nitrite and dimethyl sulfoxide (DMSO) may be used as reducible substrates for alternative energy generation pathways. Our methodology revealed a unique transcriptional profile of E. coli persisters.

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