Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and shaking rat kawasaki

Noritaka Ichinohe, Adrian Knight, Masaharu Ogawa, Toshio Ohshima, Katsuhiko Mikoshiba, Yoshihiro Yoshihara, Toshio Terashima, Kathleen S. Rockland

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The rat granular retrosplenial cortex (GRS) is a simplified cortex, with distinct stratification and, in the uppermost layers, distinct modularity. Thalamic and cortical inputs are segregated by layers and in layer 1 colocalize, respectively, with apical dendritic bundles originating from neurons in layers 2 or 5. To further investigate this organization, we turned to reelin-deficient reeler mouse and Shaking rat Kawasaki. We found that the disrupted lamination, evident in Nissl stains in these rodents, is in fact a patch-matrix mosaic of segregated afferents and dendrites. Patches consist of thalamocortical connections, visualized by vesicular glutamate transporter 2 (VGluT2) or AChE. The surrounding matrix consists of corticocortical terminations, visualized by VGluT1 or zinc. Dendrites concentrate in the matrix or patches, depending on whether they are OCAM positive (matrix) or negative (patches). In wild-type rodents and, presumably, mutants, OCAM+ structures originate from layer 5 neurons. By double labeling for dendrites (filled by Lucifer yellow in fixed slice) and OCAM immunofluorescence, we ascertained 2 populations in reeler: dendritic branches either preferred (putative layer 5 neurons) or avoided (putative supragranular neurons) the OCAM+ matrix. We conclude that input-target relationships are largely preserved in the mutant GRS and that dendrite-dendrite interactions involving OCAM influence the formation of the mosaic configuration.

Original languageEnglish
Pages (from-to)1125-1138
Number of pages14
JournalCerebral Cortex
Volume18
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

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Neurologic Mutant Mice
Dendrites
Neurons
Rodentia
Vesicular Glutamate Transport Protein 2
Fluorescent Antibody Technique
Zinc
Coloring Agents
Population

Keywords

  • Cortical layer
  • Cortical module
  • Corticocortical
  • Dendritic organization
  • OCAM
  • Thalamocortical

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Ichinohe, N., Knight, A., Ogawa, M., Ohshima, T., Mikoshiba, K., Yoshihara, Y., ... Rockland, K. S. (2008). Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and shaking rat kawasaki. Cerebral Cortex, 18(5), 1125-1138. https://doi.org/10.1093/cercor/bhm148

Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and shaking rat kawasaki. / Ichinohe, Noritaka; Knight, Adrian; Ogawa, Masaharu; Ohshima, Toshio; Mikoshiba, Katsuhiko; Yoshihara, Yoshihiro; Terashima, Toshio; Rockland, Kathleen S.

In: Cerebral Cortex, Vol. 18, No. 5, 05.2008, p. 1125-1138.

Research output: Contribution to journalArticle

Ichinohe, N, Knight, A, Ogawa, M, Ohshima, T, Mikoshiba, K, Yoshihara, Y, Terashima, T & Rockland, KS 2008, 'Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and shaking rat kawasaki', Cerebral Cortex, vol. 18, no. 5, pp. 1125-1138. https://doi.org/10.1093/cercor/bhm148
Ichinohe, Noritaka ; Knight, Adrian ; Ogawa, Masaharu ; Ohshima, Toshio ; Mikoshiba, Katsuhiko ; Yoshihara, Yoshihiro ; Terashima, Toshio ; Rockland, Kathleen S. / Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and shaking rat kawasaki. In: Cerebral Cortex. 2008 ; Vol. 18, No. 5. pp. 1125-1138.
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