Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa

Mari Suda, Maho Kodama, Yuki Oshima, Kazutoshi Yamamoto, Yoriko Nakamura, Shigeyasu Tanaka, Sakae Kikuyama, Masahisa Nakamura

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    In vertebrates, gonadal production of steroid hormones is regulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) via their receptors designated FSHR and LHR, respectively. We have shown recently that steroid hormones are synthesized in the differentiating gonad of tadpoles during sex determination in the frog Rana rugosa. To elucidate the role of gonadotropins (GTHs) and their receptors in the production of gonadal steroid hormones during sex determination, we isolated the full-length FSHβ, LHβ, FSHR and LHR cDNAs from R. rugosa and determined gonadal expression of FSHR (FSH receptor) and LHR (LH receptor) as well as brain expression of FSHβ and LHβ during sex determination in this species. The molecular structures of these four glycoproteins are conserved among different classes of vertebrates. FSHβ expression was observed at similar levels in the whole brain (including the pituitary) of tadpoles, but it showed no sexual dimorphism during gonadal sex determination. By contrast, LHβ mRNA was undetectable in the whole brain of tadpoles. FSHβ-immunopositive cells were observed in the pituitary of female tadpoles with a differentiating gonad. Furthermore, FSHR expression was significantly higher in the gonad of female tadpoles during sex determination than in that of males, whereas LHR was expressed at similar levels in males and females. The results collectively suggest that FSHR, probably in conjunction with FSH, is involved in the steroid-hormone production during female-sex determination in R. rugosa.

    Original languageEnglish
    Pages (from-to)475-486
    Number of pages12
    JournalGeneral and Comparative Endocrinology
    Volume172
    Issue number3
    DOIs
    Publication statusPublished - 2011 Jul 1

    Fingerprint

    FSH Receptors
    Ranidae
    LH Receptors
    Follicle Stimulating Hormone
    Anura
    frogs
    Up-Regulation
    follicle-stimulating hormone
    Larva
    receptors
    gender
    tadpoles
    Gonads
    Luteinizing Hormone
    steroid hormones
    luteinizing hormone
    Gonadal Steroid Hormones
    Vertebrates
    gonads
    Brain

    Keywords

    • CDNA
    • Gonad
    • Gonadotropins
    • Immunostaining
    • Pituitary
    • Rana rugosa
    • Receptors
    • Sex determination

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Suda, M., Kodama, M., Oshima, Y., Yamamoto, K., Nakamura, Y., Tanaka, S., ... Nakamura, M. (2011). Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa. General and Comparative Endocrinology, 172(3), 475-486. https://doi.org/10.1016/j.ygcen.2011.04.011

    Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa. / Suda, Mari; Kodama, Maho; Oshima, Yuki; Yamamoto, Kazutoshi; Nakamura, Yoriko; Tanaka, Shigeyasu; Kikuyama, Sakae; Nakamura, Masahisa.

    In: General and Comparative Endocrinology, Vol. 172, No. 3, 01.07.2011, p. 475-486.

    Research output: Contribution to journalArticle

    Suda, M, Kodama, M, Oshima, Y, Yamamoto, K, Nakamura, Y, Tanaka, S, Kikuyama, S & Nakamura, M 2011, 'Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa', General and Comparative Endocrinology, vol. 172, no. 3, pp. 475-486. https://doi.org/10.1016/j.ygcen.2011.04.011
    Suda, Mari ; Kodama, Maho ; Oshima, Yuki ; Yamamoto, Kazutoshi ; Nakamura, Yoriko ; Tanaka, Shigeyasu ; Kikuyama, Sakae ; Nakamura, Masahisa. / Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa. In: General and Comparative Endocrinology. 2011 ; Vol. 172, No. 3. pp. 475-486.
    @article{d189e2183c174f43b7b804670220d1ea,
    title = "Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa",
    abstract = "In vertebrates, gonadal production of steroid hormones is regulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) via their receptors designated FSHR and LHR, respectively. We have shown recently that steroid hormones are synthesized in the differentiating gonad of tadpoles during sex determination in the frog Rana rugosa. To elucidate the role of gonadotropins (GTHs) and their receptors in the production of gonadal steroid hormones during sex determination, we isolated the full-length FSHβ, LHβ, FSHR and LHR cDNAs from R. rugosa and determined gonadal expression of FSHR (FSH receptor) and LHR (LH receptor) as well as brain expression of FSHβ and LHβ during sex determination in this species. The molecular structures of these four glycoproteins are conserved among different classes of vertebrates. FSHβ expression was observed at similar levels in the whole brain (including the pituitary) of tadpoles, but it showed no sexual dimorphism during gonadal sex determination. By contrast, LHβ mRNA was undetectable in the whole brain of tadpoles. FSHβ-immunopositive cells were observed in the pituitary of female tadpoles with a differentiating gonad. Furthermore, FSHR expression was significantly higher in the gonad of female tadpoles during sex determination than in that of males, whereas LHR was expressed at similar levels in males and females. The results collectively suggest that FSHR, probably in conjunction with FSH, is involved in the steroid-hormone production during female-sex determination in R. rugosa.",
    keywords = "CDNA, Gonad, Gonadotropins, Immunostaining, Pituitary, Rana rugosa, Receptors, Sex determination",
    author = "Mari Suda and Maho Kodama and Yuki Oshima and Kazutoshi Yamamoto and Yoriko Nakamura and Shigeyasu Tanaka and Sakae Kikuyama and Masahisa Nakamura",
    year = "2011",
    month = "7",
    day = "1",
    doi = "10.1016/j.ygcen.2011.04.011",
    language = "English",
    volume = "172",
    pages = "475--486",
    journal = "General and Comparative Endocrinology",
    issn = "0016-6480",
    publisher = "Academic Press Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - Up-regulation of FSHR expression during gonadal sex determination in the frog Rana rugosa

    AU - Suda, Mari

    AU - Kodama, Maho

    AU - Oshima, Yuki

    AU - Yamamoto, Kazutoshi

    AU - Nakamura, Yoriko

    AU - Tanaka, Shigeyasu

    AU - Kikuyama, Sakae

    AU - Nakamura, Masahisa

    PY - 2011/7/1

    Y1 - 2011/7/1

    N2 - In vertebrates, gonadal production of steroid hormones is regulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) via their receptors designated FSHR and LHR, respectively. We have shown recently that steroid hormones are synthesized in the differentiating gonad of tadpoles during sex determination in the frog Rana rugosa. To elucidate the role of gonadotropins (GTHs) and their receptors in the production of gonadal steroid hormones during sex determination, we isolated the full-length FSHβ, LHβ, FSHR and LHR cDNAs from R. rugosa and determined gonadal expression of FSHR (FSH receptor) and LHR (LH receptor) as well as brain expression of FSHβ and LHβ during sex determination in this species. The molecular structures of these four glycoproteins are conserved among different classes of vertebrates. FSHβ expression was observed at similar levels in the whole brain (including the pituitary) of tadpoles, but it showed no sexual dimorphism during gonadal sex determination. By contrast, LHβ mRNA was undetectable in the whole brain of tadpoles. FSHβ-immunopositive cells were observed in the pituitary of female tadpoles with a differentiating gonad. Furthermore, FSHR expression was significantly higher in the gonad of female tadpoles during sex determination than in that of males, whereas LHR was expressed at similar levels in males and females. The results collectively suggest that FSHR, probably in conjunction with FSH, is involved in the steroid-hormone production during female-sex determination in R. rugosa.

    AB - In vertebrates, gonadal production of steroid hormones is regulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) via their receptors designated FSHR and LHR, respectively. We have shown recently that steroid hormones are synthesized in the differentiating gonad of tadpoles during sex determination in the frog Rana rugosa. To elucidate the role of gonadotropins (GTHs) and their receptors in the production of gonadal steroid hormones during sex determination, we isolated the full-length FSHβ, LHβ, FSHR and LHR cDNAs from R. rugosa and determined gonadal expression of FSHR (FSH receptor) and LHR (LH receptor) as well as brain expression of FSHβ and LHβ during sex determination in this species. The molecular structures of these four glycoproteins are conserved among different classes of vertebrates. FSHβ expression was observed at similar levels in the whole brain (including the pituitary) of tadpoles, but it showed no sexual dimorphism during gonadal sex determination. By contrast, LHβ mRNA was undetectable in the whole brain of tadpoles. FSHβ-immunopositive cells were observed in the pituitary of female tadpoles with a differentiating gonad. Furthermore, FSHR expression was significantly higher in the gonad of female tadpoles during sex determination than in that of males, whereas LHR was expressed at similar levels in males and females. The results collectively suggest that FSHR, probably in conjunction with FSH, is involved in the steroid-hormone production during female-sex determination in R. rugosa.

    KW - CDNA

    KW - Gonad

    KW - Gonadotropins

    KW - Immunostaining

    KW - Pituitary

    KW - Rana rugosa

    KW - Receptors

    KW - Sex determination

    UR - http://www.scopus.com/inward/record.url?scp=79957839441&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=79957839441&partnerID=8YFLogxK

    U2 - 10.1016/j.ygcen.2011.04.011

    DO - 10.1016/j.ygcen.2011.04.011

    M3 - Article

    C2 - 21521644

    AN - SCOPUS:79957839441

    VL - 172

    SP - 475

    EP - 486

    JO - General and Comparative Endocrinology

    JF - General and Comparative Endocrinology

    SN - 0016-6480

    IS - 3

    ER -