Upstream half of adenovirus type 2 enhancer adopts a curved DNA conformation

Takashi Ohyama, Shuichi Hashimoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The putative enhancer domain of human adenovirus type 2 (Ad2) was revealed to contain a bent DNA structure in the upstream half. By using "deletion analysis", this unusual structure was identified experimentally to span from nucleotide 194 to 269 (figures; nucleotide numbers from the left terminal nucleotide of the viral DNA). This region has almost the same nucleotide sequence as the upstream half of the enhancer and packaging region of Ad5 and, therefore, is thought to contain multiple enhancer elements for transcription and the elements required for packaging of Ad2 DNA. The bent DNA structure of this region was further characterized by analyzing temperature-dependent changes in electrophoretic mobility of the DNA fragments used for bending analysis. Ad2 enhancer provides a good system to investigate the relationship between higher order structure of DNA and transcriptional activation.

Original languageEnglish
Pages (from-to)3845-3853
Number of pages9
JournalNucleic Acids Research
Volume17
Issue number10
DOIs
Publication statusPublished - 1989 May 25
Externally publishedYes

Fingerprint

Nucleic Acid Conformation
Conformation
Adenoviridae
Conformations
DNA
Nucleotides
Packaging
Product Packaging
Human Adenoviruses
Electrophoretic mobility
Transcription
Deletion
Viral DNA
Activation
Figure
Fragment
Transcriptional Activation
Higher Order
Chemical activation
Dependent

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Upstream half of adenovirus type 2 enhancer adopts a curved DNA conformation. / Ohyama, Takashi; Hashimoto, Shuichi.

In: Nucleic Acids Research, Vol. 17, No. 10, 25.05.1989, p. 3845-3853.

Research output: Contribution to journalArticle

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