Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh, Ariella Evenzahav, Katsuyuki Kinoshita, Yoshinori Inagaki, Hiroshi Mizushima, Akira Takahashi, Tsuyoshi Hayakawa, Kazuhiko Kinosita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsB. Chance, R.R. Alfano, A. Katzir
Pages733-740
Number of pages8
Volume2979
DOIs
Publication statusPublished - 1997
Externally publishedYes
EventProceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II - San Jose, CA, United States
Duration: 1997 Feb 91997 Feb 12

Other

OtherProceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II
CountryUnited States
CitySan Jose, CA
Period97/2/997/2/12

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Keywords

  • Cellular phenomena
  • Dual-view assembly
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Framing camera
  • Frequency domain
  • Phase-resolved

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Itoh, H., Evenzahav, A., Kinoshita, K., Inagaki, Y., Mizushima, H., Takahashi, A., Hayakawa, T., & Kinosita, K. (1997). Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. In B. Chance, R. R. Alfano, & A. Katzir (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2979, pp. 733-740) https://doi.org/10.1117/12.280219