Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh; Ariella Evenzahav; Katsuyuki Kinoshita; Yoshinori Inagaki; Hiroshi Mizushima; Akira Takahashi; Tsuyoshi Hayakawa; Kazuhiko Kinosita

doi:10.1117/12.280219

Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh^*, Ariella Evenzahav, Katsuyuki Kinoshita, Yoshinori Inagaki, Hiroshi Mizushima, Akira Takahashi, Tsuyoshi Hayakawa, Kazuhiko Kinosita

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	B. Chance, R.R. Alfano, A. Katzir
Pages	733-740
Number of pages	8
Volume	2979
DOIs	https://doi.org/10.1117/12.280219
Publication status	Published - 1997
Externally published	Yes
Event	Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II - San Jose, CA, United States Duration: 1997 Feb 9 → 1997 Feb 12

Other

Other	Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II
Country/Territory	United States
City	San Jose, CA
Period	97/2/9 → 97/2/12

Keywords

Cellular phenomena
Dual-view assembly
Fluorescence lifetime imaging microscopy (FLIM)
Framing camera
Frequency domain
Phase-resolved

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

Access to Document

10.1117/12.280219

Cite this

Itoh, H., Evenzahav, A., Kinoshita, K., Inagaki, Y., Mizushima, H., Takahashi, A., Hayakawa, T., & Kinosita, K. (1997). Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. In B. Chance, R. R. Alfano, & A. Katzir (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2979, pp. 733-740) https://doi.org/10.1117/12.280219

Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. / Itoh, Hiroyasu; Evenzahav, Ariella; Kinoshita, Katsuyuki et al.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / B. Chance; R.R. Alfano; A. Katzir. Vol. 2979 1997. p. 733-740.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Itoh, H, Evenzahav, A, Kinoshita, K, Inagaki, Y, Mizushima, H, Takahashi, A, Hayakawa, T & Kinosita, K 1997, Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. in B Chance, RR Alfano & A Katzir (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2979, pp. 733-740, Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II, San Jose, CA, United States, 97/2/9. https://doi.org/10.1117/12.280219

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abstract = "A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.",

keywords = "Cellular phenomena, Dual-view assembly, Fluorescence lifetime imaging microscopy (FLIM), Framing camera, Frequency domain, Phase-resolved",

author = "Hiroyasu Itoh and Ariella Evenzahav and Katsuyuki Kinoshita and Yoshinori Inagaki and Hiroshi Mizushima and Akira Takahashi and Tsuyoshi Hayakawa and Kazuhiko Kinosita",

year = "1997",

doi = "10.1117/12.280219",

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volume = "2979",

pages = "733--740",

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AU - Itoh, Hiroyasu

AU - Evenzahav, Ariella

AU - Kinoshita, Katsuyuki

AU - Inagaki, Yoshinori

AU - Mizushima, Hiroshi

AU - Takahashi, Akira

AU - Hayakawa, Tsuyoshi

AU - Kinosita, Kazuhiko

PY - 1997

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N2 - A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

AB - A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

KW - Cellular phenomena

KW - Dual-view assembly

KW - Fluorescence lifetime imaging microscopy (FLIM)

KW - Framing camera

KW - Frequency domain

KW - Phase-resolved

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BT - Proceedings of SPIE - The International Society for Optical Engineering

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Y2 - 9 February 1997 through 12 February 1997

ER -

Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

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