Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)571-575
Number of pages5
JournalBiotechnology and Bioengineering
Volume68
Issue number5
DOIs
Publication statusPublished - 2000 Jun 5
Externally publishedYes

Fingerprint

Anion Exchange Resins
Antibodies
Systemic Lupus Erythematosus
Alkaline Phosphatase
Anti-Idiotypic Antibodies
Ion exchange
DNA
Negative ions
Resins
Antinuclear Antibodies
Isoelectric Point
Luminescence
Serum
Immunoassay
Antibody Formation
Phosphatases
Assays

Keywords

  • Alkaline phosphatase conjugated DNA
  • Anion exchange capillary column
  • Anti-dsDNA antibody
  • Isoelectric point
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum. / Lim, Tae Kyu; Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Biotechnology and Bioengineering, Vol. 68, No. 5, 05.06.2000, p. 571-575.

Research output: Contribution to journalArticle

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