Use of folded micromachined pillar array column with low-dispersion turns for pressure-driven liquid chromatography

Chiaki Aoyama, Akira Saeki, Masao Noguchi, Yoshitaka Shirasaki, Shuichi Shoji, Takashi Funatsu, Jun Mizuno, Makoto Tsunoda

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In this study, we show for the first time that the separation efficiency of a pillar array column under pressure-driven liquid chromatography (LC) conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. With the low-dispersion-turn geometry, a column with a length and width of 110 mm and 400 μ m, respectively, could be fabricated on a 20 x 20 mm microchip. Under nonretained conditions, the solute bands obtained for fluorescent compounds remained almost unchanged even after passing through the lowdispersion turns; however, significant skewing of the solute bands was observed in the case of constant-radius turns. Two coumarin dyes were well resolved under reversed-phase conditions, and a maximum theoretical plate number of 8000 was obtained. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s. These results indicated that the separation efficiency of microchip chromatography could be significantly improved using a long separation channel with low-dispersion turns.

Original languageEnglish
Pages (from-to)1420-1426
Number of pages7
JournalAnalytical chemistry
Volume82
Issue number4
DOIs
Publication statusPublished - 2010 Feb 15

ASJC Scopus subject areas

  • Analytical Chemistry

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