Use of hydroxyapatite small crystals as drug carrier

Seisuke Kano, Atsushi Yamazaki, Ryohei Otsuka, Masaru Akao, Hideki Aoki

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Hydroxyapatite small crystals(HAp-sol) applied as a drug carrier are being developed. The HAp-sol was synthesized by a wet method and amounts of drug adsorbed onto the hydroxyapatite small crystals were measured. The HAp-sol was characterized by X-ray powder diffraction, transmission electron microscopy, and infrared absorption. From the TEM observation, the crystal had a stick-like shape (average length was 730 Å and width was 140 Å). The amount of drug adsorbed onto the crystals was measured by an ultraviolet-visible radiation spectrophotometer. The drugs used were doxorubicin hydrochloride, mitomycin C, and fluorouracil (Kyowa Hakkou) which are glycoside antibiotics. An adsorption curve as a function of time, adsorption isotherm, and eluted amount of adsorbed drug were measured. Investigation of the adsorption was carried out at pH 7.50 (1.0 mmol/1 phosphoric buffer solution) and incubated at 37°C. The concentration of HAp-sol was 5 mg/ml. The saturation time of doxorubicin-hydrochloride-adsorption onto HAp-sol required 2 hours. The amount of doxorubicin hydrochloride saturated adsorption was 0.2 mg per 1 mg HAp-sol. In the measurement of the eluted amount of doxorubicin hydrochloride, half of the saturated adsorbed drug was eluted into the phosphoric buffer solution. This result indicates that HAp-sol adsorbed with doxorubicin hydrochloride is useful as a drug delivery system.

    Original languageEnglish
    Pages (from-to)467-471
    Number of pages5
    JournalDrug Delivery System
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - 1993

    Fingerprint

    Drug Carriers
    Polymethyl Methacrylate
    Durapatite
    Doxorubicin
    Adsorption
    Pharmaceutical Preparations
    Buffers
    Powder Diffraction
    Mitomycin
    Drug Delivery Systems
    Glycosides
    Transmission Electron Microscopy
    X-Ray Diffraction
    Fluorouracil
    Observation
    Anti-Bacterial Agents
    Light

    Keywords

    • adsorption
    • antibiotics
    • drug carrier
    • HAp-sol
    • hydroxypatite

    ASJC Scopus subject areas

    • Pharmaceutical Science

    Cite this

    Use of hydroxyapatite small crystals as drug carrier. / Kano, Seisuke; Yamazaki, Atsushi; Otsuka, Ryohei; Akao, Masaru; Aoki, Hideki.

    In: Drug Delivery System, Vol. 8, No. 6, 1993, p. 467-471.

    Research output: Contribution to journalArticle

    Kano, Seisuke ; Yamazaki, Atsushi ; Otsuka, Ryohei ; Akao, Masaru ; Aoki, Hideki. / Use of hydroxyapatite small crystals as drug carrier. In: Drug Delivery System. 1993 ; Vol. 8, No. 6. pp. 467-471.
    @article{efd83c55c67f4b65985c0cffa35460a9,
    title = "Use of hydroxyapatite small crystals as drug carrier",
    abstract = "Hydroxyapatite small crystals(HAp-sol) applied as a drug carrier are being developed. The HAp-sol was synthesized by a wet method and amounts of drug adsorbed onto the hydroxyapatite small crystals were measured. The HAp-sol was characterized by X-ray powder diffraction, transmission electron microscopy, and infrared absorption. From the TEM observation, the crystal had a stick-like shape (average length was 730 {\AA} and width was 140 {\AA}). The amount of drug adsorbed onto the crystals was measured by an ultraviolet-visible radiation spectrophotometer. The drugs used were doxorubicin hydrochloride, mitomycin C, and fluorouracil (Kyowa Hakkou) which are glycoside antibiotics. An adsorption curve as a function of time, adsorption isotherm, and eluted amount of adsorbed drug were measured. Investigation of the adsorption was carried out at pH 7.50 (1.0 mmol/1 phosphoric buffer solution) and incubated at 37°C. The concentration of HAp-sol was 5 mg/ml. The saturation time of doxorubicin-hydrochloride-adsorption onto HAp-sol required 2 hours. The amount of doxorubicin hydrochloride saturated adsorption was 0.2 mg per 1 mg HAp-sol. In the measurement of the eluted amount of doxorubicin hydrochloride, half of the saturated adsorbed drug was eluted into the phosphoric buffer solution. This result indicates that HAp-sol adsorbed with doxorubicin hydrochloride is useful as a drug delivery system.",
    keywords = "adsorption, antibiotics, drug carrier, HAp-sol, hydroxypatite",
    author = "Seisuke Kano and Atsushi Yamazaki and Ryohei Otsuka and Masaru Akao and Hideki Aoki",
    year = "1993",
    doi = "10.2745/dds.8.467",
    language = "English",
    volume = "8",
    pages = "467--471",
    journal = "Drug Delivery System",
    issn = "0913-5006",
    publisher = "Japan Society of Drug Delivery System",
    number = "6",

    }

    TY - JOUR

    T1 - Use of hydroxyapatite small crystals as drug carrier

    AU - Kano, Seisuke

    AU - Yamazaki, Atsushi

    AU - Otsuka, Ryohei

    AU - Akao, Masaru

    AU - Aoki, Hideki

    PY - 1993

    Y1 - 1993

    N2 - Hydroxyapatite small crystals(HAp-sol) applied as a drug carrier are being developed. The HAp-sol was synthesized by a wet method and amounts of drug adsorbed onto the hydroxyapatite small crystals were measured. The HAp-sol was characterized by X-ray powder diffraction, transmission electron microscopy, and infrared absorption. From the TEM observation, the crystal had a stick-like shape (average length was 730 Å and width was 140 Å). The amount of drug adsorbed onto the crystals was measured by an ultraviolet-visible radiation spectrophotometer. The drugs used were doxorubicin hydrochloride, mitomycin C, and fluorouracil (Kyowa Hakkou) which are glycoside antibiotics. An adsorption curve as a function of time, adsorption isotherm, and eluted amount of adsorbed drug were measured. Investigation of the adsorption was carried out at pH 7.50 (1.0 mmol/1 phosphoric buffer solution) and incubated at 37°C. The concentration of HAp-sol was 5 mg/ml. The saturation time of doxorubicin-hydrochloride-adsorption onto HAp-sol required 2 hours. The amount of doxorubicin hydrochloride saturated adsorption was 0.2 mg per 1 mg HAp-sol. In the measurement of the eluted amount of doxorubicin hydrochloride, half of the saturated adsorbed drug was eluted into the phosphoric buffer solution. This result indicates that HAp-sol adsorbed with doxorubicin hydrochloride is useful as a drug delivery system.

    AB - Hydroxyapatite small crystals(HAp-sol) applied as a drug carrier are being developed. The HAp-sol was synthesized by a wet method and amounts of drug adsorbed onto the hydroxyapatite small crystals were measured. The HAp-sol was characterized by X-ray powder diffraction, transmission electron microscopy, and infrared absorption. From the TEM observation, the crystal had a stick-like shape (average length was 730 Å and width was 140 Å). The amount of drug adsorbed onto the crystals was measured by an ultraviolet-visible radiation spectrophotometer. The drugs used were doxorubicin hydrochloride, mitomycin C, and fluorouracil (Kyowa Hakkou) which are glycoside antibiotics. An adsorption curve as a function of time, adsorption isotherm, and eluted amount of adsorbed drug were measured. Investigation of the adsorption was carried out at pH 7.50 (1.0 mmol/1 phosphoric buffer solution) and incubated at 37°C. The concentration of HAp-sol was 5 mg/ml. The saturation time of doxorubicin-hydrochloride-adsorption onto HAp-sol required 2 hours. The amount of doxorubicin hydrochloride saturated adsorption was 0.2 mg per 1 mg HAp-sol. In the measurement of the eluted amount of doxorubicin hydrochloride, half of the saturated adsorbed drug was eluted into the phosphoric buffer solution. This result indicates that HAp-sol adsorbed with doxorubicin hydrochloride is useful as a drug delivery system.

    KW - adsorption

    KW - antibiotics

    KW - drug carrier

    KW - HAp-sol

    KW - hydroxypatite

    UR - http://www.scopus.com/inward/record.url?scp=17444412580&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=17444412580&partnerID=8YFLogxK

    U2 - 10.2745/dds.8.467

    DO - 10.2745/dds.8.467

    M3 - Article

    AN - SCOPUS:17444412580

    VL - 8

    SP - 467

    EP - 471

    JO - Drug Delivery System

    JF - Drug Delivery System

    SN - 0913-5006

    IS - 6

    ER -