Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization

Tadashi Matsunaga, Shinji Kamiya

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

We report the novel use of magnetic particles isolated from magnetotactic bacteria. Magnetotactic bacteria were collected from enriched sludge by use of a magnetic harvesting apparatus. Magnetic particles separated from magnetotactic bacteria were shown to be pure magnetite. Glucose oxidase and uricase were immobilized on magnetic particles. The activity of glucose oxidase immobilized on biogenic magnetites was 40 times that immobilized on artificial magnetites or Zn-ferrite particles. Both glucose oxidase and uricase coupled with biogenic magnetic particles retained their activities when they were reused 5 times.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume26
Issue number4
DOIs
Publication statusPublished - 1987 Jul
Externally publishedYes

Fingerprint

Ferrosoferric Oxide
Enzyme immobilization
Glucose Oxidase
Glucose oxidase
Magnetite
Urate Oxidase
Immobilization
Bacteria
Enzymes
Sewage
Ferrite

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization. / Matsunaga, Tadashi; Kamiya, Shinji.

In: Applied Microbiology and Biotechnology, Vol. 26, No. 4, 07.1987, p. 328-332.

Research output: Contribution to journalArticle

@article{140f7702263c400f9e0667105b7a026c,
title = "Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization",
abstract = "We report the novel use of magnetic particles isolated from magnetotactic bacteria. Magnetotactic bacteria were collected from enriched sludge by use of a magnetic harvesting apparatus. Magnetic particles separated from magnetotactic bacteria were shown to be pure magnetite. Glucose oxidase and uricase were immobilized on magnetic particles. The activity of glucose oxidase immobilized on biogenic magnetites was 40 times that immobilized on artificial magnetites or Zn-ferrite particles. Both glucose oxidase and uricase coupled with biogenic magnetic particles retained their activities when they were reused 5 times.",
author = "Tadashi Matsunaga and Shinji Kamiya",
year = "1987",
month = "7",
doi = "10.1007/BF00256663",
language = "English",
volume = "26",
pages = "328--332",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization

AU - Matsunaga, Tadashi

AU - Kamiya, Shinji

PY - 1987/7

Y1 - 1987/7

N2 - We report the novel use of magnetic particles isolated from magnetotactic bacteria. Magnetotactic bacteria were collected from enriched sludge by use of a magnetic harvesting apparatus. Magnetic particles separated from magnetotactic bacteria were shown to be pure magnetite. Glucose oxidase and uricase were immobilized on magnetic particles. The activity of glucose oxidase immobilized on biogenic magnetites was 40 times that immobilized on artificial magnetites or Zn-ferrite particles. Both glucose oxidase and uricase coupled with biogenic magnetic particles retained their activities when they were reused 5 times.

AB - We report the novel use of magnetic particles isolated from magnetotactic bacteria. Magnetotactic bacteria were collected from enriched sludge by use of a magnetic harvesting apparatus. Magnetic particles separated from magnetotactic bacteria were shown to be pure magnetite. Glucose oxidase and uricase were immobilized on magnetic particles. The activity of glucose oxidase immobilized on biogenic magnetites was 40 times that immobilized on artificial magnetites or Zn-ferrite particles. Both glucose oxidase and uricase coupled with biogenic magnetic particles retained their activities when they were reused 5 times.

UR - http://www.scopus.com/inward/record.url?scp=0023195035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023195035&partnerID=8YFLogxK

U2 - 10.1007/BF00256663

DO - 10.1007/BF00256663

M3 - Article

AN - SCOPUS:0023195035

VL - 26

SP - 328

EP - 332

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 4

ER -