Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'

G. Nakagawa, Y. Ebie, Satoshi Tsuneda, M. Matsumura, Y. Inamori

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    10 Citations (Scopus)

    Abstract

    The aim of this study was to examine the relationship between ammonia oxidizing bacterial populations and biological nitrogen removal in a small on-site domestic wastewater treatment system "Johkasou". The population dynamics of ammonia oxidizing bacteria (AOB) in six full-scale advanced Johkasous was surveyed using real-time PCR assay over a period of one year. These Johkasous were selected to compare the AOB populations in different treatment performance. When the effluent NH4-N concentration was higher than 2 mg L-1, it was difficult to meet the effluent standard of advanced Johkasous (T-N 10 mg L-1). In contrast, the nitrogen removal efficiency was hardly affected by nitrite oxidation and denitrification in these systems. In other words, ammonia oxidation was a rate-limiting step. Furthermore, we focused on the relationship between NH4-N loading per AOB cell and nitrogen removal. Real time PCR monitoring results demonstrated that it is important to regulate NH4-N loading per AOB cell below 210 pg cell-1 day-1 to meet the effluent standard of advanced Johkasou. It is considered that NH4-N loading per AOB cell is a useful parameter for determining suitable nitrogen loading and small decentralized system design.

    Original languageEnglish
    Title of host publicationWater Science and Technology
    Pages203-210
    Number of pages8
    Volume55
    Edition7
    DOIs
    Publication statusPublished - 2007

    Publication series

    NameWater Science and Technology
    Number7
    Volume55
    ISSN (Print)02731223

    Fingerprint

    ammonia
    nitrogen
    bacterium
    effluent
    oxidation
    removal
    nitrite
    denitrification
    population dynamics
    assay
    monitoring

    Keywords

    • Ammonia oxidizing bacteria
    • Biological nitrogen removal
    • Domestic wastewater
    • Johkasou
    • Real-time PCR
    • Small decentralized system

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Nakagawa, G., Ebie, Y., Tsuneda, S., Matsumura, M., & Inamori, Y. (2007). Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'. In Water Science and Technology (7 ed., Vol. 55, pp. 203-210). (Water Science and Technology; Vol. 55, No. 7). https://doi.org/10.2166/wst.2007.146

    Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'. / Nakagawa, G.; Ebie, Y.; Tsuneda, Satoshi; Matsumura, M.; Inamori, Y.

    Water Science and Technology. Vol. 55 7. ed. 2007. p. 203-210 (Water Science and Technology; Vol. 55, No. 7).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Nakagawa, G, Ebie, Y, Tsuneda, S, Matsumura, M & Inamori, Y 2007, Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'. in Water Science and Technology. 7 edn, vol. 55, Water Science and Technology, no. 7, vol. 55, pp. 203-210. https://doi.org/10.2166/wst.2007.146
    Nakagawa, G. ; Ebie, Y. ; Tsuneda, Satoshi ; Matsumura, M. ; Inamori, Y. / Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'. Water Science and Technology. Vol. 55 7. ed. 2007. pp. 203-210 (Water Science and Technology; 7).
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