Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'

G. Nakagawa, Y. Ebie, S. Tsuneda, M. Matsumura, Y. Inamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

The aim of this study was to examine the relationship between ammonia oxidizing bacterial populations and biological nitrogen removal in a small on-site domestic wastewater treatment system "Johkasou". The population dynamics of ammonia oxidizing bacteria (AOB) in six full-scale advanced Johkasous was surveyed using real-time PCR assay over a period of one year. These Johkasous were selected to compare the AOB populations in different treatment performance. When the effluent NH4-N concentration was higher than 2 mg L-1, it was difficult to meet the effluent standard of advanced Johkasous (T-N 10 mg L-1). In contrast, the nitrogen removal efficiency was hardly affected by nitrite oxidation and denitrification in these systems. In other words, ammonia oxidation was a rate-limiting step. Furthermore, we focused on the relationship between NH4-N loading per AOB cell and nitrogen removal. Real time PCR monitoring results demonstrated that it is important to regulate NH4-N loading per AOB cell below 210 pg cell-1 day-1 to meet the effluent standard of advanced Johkasou. It is considered that NH4-N loading per AOB cell is a useful parameter for determining suitable nitrogen loading and small decentralized system design.

Original languageEnglish
Title of host publicationSmall Water and Wastewater Systems VII
EditorsS.G. Martinez
Pages203-210
Number of pages8
Edition7
DOIs
Publication statusPublished - 2007 May 22

Publication series

NameWater Science and Technology
Number7
Volume55
ISSN (Print)0273-1223

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Keywords

  • Ammonia oxidizing bacteria
  • Biological nitrogen removal
  • Domestic wastewater
  • Johkasou
  • Real-time PCR
  • Small decentralized system

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

Cite this

Nakagawa, G., Ebie, Y., Tsuneda, S., Matsumura, M., & Inamori, Y. (2007). Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'. In S. G. Martinez (Ed.), Small Water and Wastewater Systems VII (7 ed., pp. 203-210). (Water Science and Technology; Vol. 55, No. 7). https://doi.org/10.2166/wst.2007.146