Abstract
The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.
| Original language | English |
|---|---|
| Title of host publication | American Society of Mechanical Engineers, Bioengineering Division (Publication) BED |
| Place of Publication | Fairfield, NJ, United States |
| Publisher | ASME |
| Pages | 57-58 |
| Number of pages | 2 |
| Volume | 44 |
| ISBN (Print) | 0791816435 |
| Publication status | Published - 1999 |
| Externally published | Yes |
| Event | Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA Duration: 1999 Nov 14 → 1999 Nov 19 |
Other
| Other | Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) |
|---|---|
| City | Nashville, TN, USA |
| Period | 99/11/14 → 99/11/19 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Using encapsulation to improve the viability of cryopreserved cells. / Matsumoto, Yoshifumi; Morinaga, Yukihiro; Ujihira, Masanobu; Oka, Kotaro; Tanishita, Kazuo.
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44 Fairfield, NJ, United States : ASME, 1999. p. 57-58.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Using encapsulation to improve the viability of cryopreserved cells
AU - Matsumoto, Yoshifumi
AU - Morinaga, Yukihiro
AU - Ujihira, Masanobu
AU - Oka, Kotaro
AU - Tanishita, Kazuo
PY - 1999
Y1 - 1999
N2 - The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.
AB - The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.
UR - http://www.scopus.com/inward/record.url?scp=0033294188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033294188&partnerID=8YFLogxK
M3 - Chapter
SN - 0791816435
VL - 44
SP - 57
EP - 58
BT - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
PB - ASME
CY - Fairfield, NJ, United States
ER -