Using encapsulation to improve the viability of cryopreserved cells

Yoshifumi Matsumoto, Yukihiro Morinaga, Masanobu Ujihira, Kotaro Oka, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.

Original languageEnglish
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Place of PublicationFairfield, NJ, United States
PublisherASME
Pages57-58
Number of pages2
Volume44
ISBN (Print)0791816435
Publication statusPublished - 1999
Externally publishedYes
EventAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA
Duration: 1999 Nov 141999 Nov 19

Other

OtherAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition)
CityNashville, TN, USA
Period99/11/1499/11/19

Fingerprint

Latent heat
Alginate
Encapsulation
Freezing
Cells
Cooling
Ice
Rats
Differential scanning calorimetry
Assays
Dyes
Membranes
Crystals
Temperature
Dopamine

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Matsumoto, Y., Morinaga, Y., Ujihira, M., Oka, K., & Tanishita, K. (1999). Using encapsulation to improve the viability of cryopreserved cells. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (Vol. 44, pp. 57-58). Fairfield, NJ, United States: ASME.

Using encapsulation to improve the viability of cryopreserved cells. / Matsumoto, Yoshifumi; Morinaga, Yukihiro; Ujihira, Masanobu; Oka, Kotaro; Tanishita, Kazuo.

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44 Fairfield, NJ, United States : ASME, 1999. p. 57-58.

Research output: Chapter in Book/Report/Conference proceedingChapter

Matsumoto, Y, Morinaga, Y, Ujihira, M, Oka, K & Tanishita, K 1999, Using encapsulation to improve the viability of cryopreserved cells. in American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. vol. 44, ASME, Fairfield, NJ, United States, pp. 57-58, Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition), Nashville, TN, USA, 99/11/14.
Matsumoto Y, Morinaga Y, Ujihira M, Oka K, Tanishita K. Using encapsulation to improve the viability of cryopreserved cells. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44. Fairfield, NJ, United States: ASME. 1999. p. 57-58
Matsumoto, Yoshifumi ; Morinaga, Yukihiro ; Ujihira, Masanobu ; Oka, Kotaro ; Tanishita, Kazuo. / Using encapsulation to improve the viability of cryopreserved cells. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44 Fairfield, NJ, United States : ASME, 1999. pp. 57-58
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