Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size

Jun Takagi, Takeshi Itabashi, Kazuya Suzuki, Tarun M. Kapoor, Yuta Shimamoto, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles thatself-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density

    Original languageEnglish
    Pages (from-to)44-50
    Number of pages7
    JournalCell Reports
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - 2013 Oct 17

    Fingerprint

    Micromanipulation
    Spindle Apparatus
    Microtubules
    Vertebrates
    Poles
    Depolymerization
    Microscopic examination
    Polymerization
    Chemical analysis
    Xenopus
    Ovum
    Microscopy

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Takagi, J., Itabashi, T., Suzuki, K., Kapoor, T. M., Shimamoto, Y., & Ishiwata, S. (2013). Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size. Cell Reports, 5(1), 44-50. https://doi.org/10.1016/j.celrep.2013.09.021

    Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size. / Takagi, Jun; Itabashi, Takeshi; Suzuki, Kazuya; Kapoor, Tarun M.; Shimamoto, Yuta; Ishiwata, Shin'ichi.

    In: Cell Reports, Vol. 5, No. 1, 17.10.2013, p. 44-50.

    Research output: Contribution to journalArticle

    Takagi, J, Itabashi, T, Suzuki, K, Kapoor, TM, Shimamoto, Y & Ishiwata, S 2013, 'Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size', Cell Reports, vol. 5, no. 1, pp. 44-50. https://doi.org/10.1016/j.celrep.2013.09.021
    Takagi J, Itabashi T, Suzuki K, Kapoor TM, Shimamoto Y, Ishiwata S. Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size. Cell Reports. 2013 Oct 17;5(1):44-50. https://doi.org/10.1016/j.celrep.2013.09.021
    Takagi, Jun ; Itabashi, Takeshi ; Suzuki, Kazuya ; Kapoor, Tarun M. ; Shimamoto, Yuta ; Ishiwata, Shin'ichi. / Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size. In: Cell Reports. 2013 ; Vol. 5, No. 1. pp. 44-50.
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