Abstract
The feasibility of using thermophysical properties to evaluate extracellular freezing processes favorable for cryopreservation was demonstrated by determining the correlation between the thermal state and the viability of cells and tissue. We measured the effective thermal conductivity of Medaka embryos (Oryzias latipes) during extracellular freezing using the self-heated thermistor technique. The insulated test chamber (8x8x12 mm) had a 2.5-mm-diameter thermistor bead installed in the bottom. The effective thermal conductivity was measured at -10°C for various cooling rates (from 0.1 to 10°C/min) for embryos in 1.92 mol/kg DMSO, and measured from 6 to -20°C for a cooling rate of 1°C/min for embryos in 0 - 2.56 mol/kg DMSO. We used the hatching rate to evaluate the viability of the embryos. The effective thermal conductivity was strongly correlated with the hatching rate, increasing as the hatching rate decreased. This demonstrates that thermophysical properties of biological materials can be used as indices for evaluating the freezing process of such materials.
| Original language | English |
|---|---|
| Pages (from-to) | 768-776 |
| Number of pages | 9 |
| Journal | JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing |
| Volume | 42 |
| Issue number | 3 |
| Publication status | Published - 1999 Sep |
| Externally published | Yes |
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Keywords
- Biological Engineering
- Cryopreservation
- Extracellular Freezing
- Medaka Embryos
- Medical Engineering
- Thermophysical Property
ASJC Scopus subject areas
- Industrial and Manufacturing Engineering
- Mechanical Engineering
Cite this
Using the effective thermal conductivity to evaluate the freezing process in Medaka embryos suspension. / Aizawa, Naoki; Shimamoto, Nobusuke; Ujihira, Masanobu; Tanishita, Kazuo.
In: JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing, Vol. 42, No. 3, 09.1999, p. 768-776.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Using the effective thermal conductivity to evaluate the freezing process in Medaka embryos suspension
AU - Aizawa, Naoki
AU - Shimamoto, Nobusuke
AU - Ujihira, Masanobu
AU - Tanishita, Kazuo
PY - 1999/9
Y1 - 1999/9
N2 - The feasibility of using thermophysical properties to evaluate extracellular freezing processes favorable for cryopreservation was demonstrated by determining the correlation between the thermal state and the viability of cells and tissue. We measured the effective thermal conductivity of Medaka embryos (Oryzias latipes) during extracellular freezing using the self-heated thermistor technique. The insulated test chamber (8x8x12 mm) had a 2.5-mm-diameter thermistor bead installed in the bottom. The effective thermal conductivity was measured at -10°C for various cooling rates (from 0.1 to 10°C/min) for embryos in 1.92 mol/kg DMSO, and measured from 6 to -20°C for a cooling rate of 1°C/min for embryos in 0 - 2.56 mol/kg DMSO. We used the hatching rate to evaluate the viability of the embryos. The effective thermal conductivity was strongly correlated with the hatching rate, increasing as the hatching rate decreased. This demonstrates that thermophysical properties of biological materials can be used as indices for evaluating the freezing process of such materials.
AB - The feasibility of using thermophysical properties to evaluate extracellular freezing processes favorable for cryopreservation was demonstrated by determining the correlation between the thermal state and the viability of cells and tissue. We measured the effective thermal conductivity of Medaka embryos (Oryzias latipes) during extracellular freezing using the self-heated thermistor technique. The insulated test chamber (8x8x12 mm) had a 2.5-mm-diameter thermistor bead installed in the bottom. The effective thermal conductivity was measured at -10°C for various cooling rates (from 0.1 to 10°C/min) for embryos in 1.92 mol/kg DMSO, and measured from 6 to -20°C for a cooling rate of 1°C/min for embryos in 0 - 2.56 mol/kg DMSO. We used the hatching rate to evaluate the viability of the embryos. The effective thermal conductivity was strongly correlated with the hatching rate, increasing as the hatching rate decreased. This demonstrates that thermophysical properties of biological materials can be used as indices for evaluating the freezing process of such materials.
KW - Biological Engineering
KW - Cryopreservation
KW - Extracellular Freezing
KW - Medaka Embryos
KW - Medical Engineering
KW - Thermophysical Property
UR - http://www.scopus.com/inward/record.url?scp=0033190511&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033190511&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033190511
VL - 42
SP - 768
EP - 776
JO - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
JF - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
SN - 1344-7653
IS - 3
ER -