Utilizing Viral Nanoparticle/Dendron Hybrid Conjugates in Photodynamic Therapy for Dual Delivery to Macrophages and Cancer Cells

Amy M. Wen, Karin L. Lee, Pengfei Cao, Katrina Pangilinan, Bradley L. Carpenter, Patricia Lam, Frank A. Veliz, Reza A. Ghiladi, Rigoberto C. Advincula, Nicole F. Steinmetz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Photodynamic therapy (PDT) is a promising avenue for greater treatment efficacy of highly resistant and aggressive melanoma. Through photosensitizer attachment to nanoparticles, specificity of delivery can be conferred to further reduce potential side effects. While the main focus of PDT is the destruction of cancer cells, additional targeting of tumor-associated macrophages also present in the tumor microenvironment could further enhance treatment by eliminating their role in processes such as invasion, metastasis, and immunosuppression. In this study, we investigated PDT of macrophages and tumor cells through delivery using the natural noninfectious nanoparticle cowpea mosaic virus (CPMV), which has been shown to have specificity for the immunosuppressive subpopulation of macrophages and also targets cancer cells. We further explored conjugation of CPMV/dendron hybrids in order to improve the drug loading capacity of the nanocarrier. Overall, we demonstrated effective elimination of both macrophage and tumor cells at low micromolar concentrations of the photosensitizer when delivered with the CPMV bioconjugate, thereby potentially improving melanoma treatment.

Original languageEnglish
Pages (from-to)1227-1235
Number of pages9
JournalBioconjugate Chemistry
Volume27
Issue number5
DOIs
Publication statusPublished - 2016 May 18
Externally publishedYes

Fingerprint

Photodynamic therapy
Macrophages
Photochemotherapy
Comovirus
Nanoparticles
Tumors
Viruses
Cells
Photosensitizing Agents
Photosensitizers
Neoplasms
Melanoma
Immunosuppressive Agents
Tumor Microenvironment
Immunosuppression
dendron
Neoplasm Metastasis
Pharmaceutical Preparations
Therapeutics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Utilizing Viral Nanoparticle/Dendron Hybrid Conjugates in Photodynamic Therapy for Dual Delivery to Macrophages and Cancer Cells. / Wen, Amy M.; Lee, Karin L.; Cao, Pengfei; Pangilinan, Katrina; Carpenter, Bradley L.; Lam, Patricia; Veliz, Frank A.; Ghiladi, Reza A.; Advincula, Rigoberto C.; Steinmetz, Nicole F.

In: Bioconjugate Chemistry, Vol. 27, No. 5, 18.05.2016, p. 1227-1235.

Research output: Contribution to journalArticle

Wen, AM, Lee, KL, Cao, P, Pangilinan, K, Carpenter, BL, Lam, P, Veliz, FA, Ghiladi, RA, Advincula, RC & Steinmetz, NF 2016, 'Utilizing Viral Nanoparticle/Dendron Hybrid Conjugates in Photodynamic Therapy for Dual Delivery to Macrophages and Cancer Cells', Bioconjugate Chemistry, vol. 27, no. 5, pp. 1227-1235. https://doi.org/10.1021/acs.bioconjchem.6b00075
Wen, Amy M. ; Lee, Karin L. ; Cao, Pengfei ; Pangilinan, Katrina ; Carpenter, Bradley L. ; Lam, Patricia ; Veliz, Frank A. ; Ghiladi, Reza A. ; Advincula, Rigoberto C. ; Steinmetz, Nicole F. / Utilizing Viral Nanoparticle/Dendron Hybrid Conjugates in Photodynamic Therapy for Dual Delivery to Macrophages and Cancer Cells. In: Bioconjugate Chemistry. 2016 ; Vol. 27, No. 5. pp. 1227-1235.
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