UV-A-induced expression of GroEL in the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600

Akira Yamazawa, Haruko Takeyama, Daisuke Takeda, Tadashi Matsunaga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The authors have examined the response to UV-A irradiation of the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600, which produces the UV-A-absorbing compound biopterin glucoside. The expression of a 60 kDa protein was markedly induced at 500 min after UV-A irradiation. This protein was identified by N-terminal amino acid sequence analysis as GroEL. Northern blot analysis demonstrated that GroEL synthesis was controlled by UV-A at the transcriptional level. A CIRCE element and a putative SOS consensus sequence were found upstream of the groESL operon, overlapping two putative promoter sequences. Primer extension analysis revealed that groESL transcription in UV-A-induced cells starts from the proximal promoter overlapped by the SOS consensus sequence. This indicates that an SOS response regulation is instrumental in UV-A-induced GroEL expression of Oscillatoria sp. NKBG 091600. Furthermore, this UV-A-inducible GroEL may function to upregulate biopterin glucoside biosynthesis, thereby allowing growth under UV-A irradiation.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalMicrobiology
Volume145
Issue number4
Publication statusPublished - 1999 Apr
Externally publishedYes

Fingerprint

Oscillatoria
Biopterin
Consensus Sequence
Glucosides
Cyanobacteria
Protein Sequence Analysis
Operon
Northern Blotting
Proteins
Up-Regulation
Growth

Keywords

  • Biopterin glucoside
  • GroEL
  • Marine cyanobacterium Oscillatoria sp.
  • Stress reponse
  • UV-A resistance

ASJC Scopus subject areas

  • Microbiology

Cite this

UV-A-induced expression of GroEL in the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600. / Yamazawa, Akira; Takeyama, Haruko; Takeda, Daisuke; Matsunaga, Tadashi.

In: Microbiology, Vol. 145, No. 4, 04.1999, p. 949-954.

Research output: Contribution to journalArticle

Yamazawa, Akira ; Takeyama, Haruko ; Takeda, Daisuke ; Matsunaga, Tadashi. / UV-A-induced expression of GroEL in the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600. In: Microbiology. 1999 ; Vol. 145, No. 4. pp. 949-954.
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