Abstract
The authors have examined the response to UV-A irradiation of the UV-A-resistant marine cyanobacterium Oscillatoria sp. NKBG 091600, which produces the UV-A-absorbing compound biopterin glucoside. The expression of a 60 kDa protein was markedly induced at 500 min after UV-A irradiation. This protein was identified by N-terminal amino acid sequence analysis as GroEL. Northern blot analysis demonstrated that GroEL synthesis was controlled by UV-A at the transcriptional level. A CIRCE element and a putative SOS consensus sequence were found upstream of the groESL operon, overlapping two putative promoter sequences. Primer extension analysis revealed that groESL transcription in UV-A-induced cells starts from the proximal promoter overlapped by the SOS consensus sequence. This indicates that an SOS response regulation is instrumental in UV-A-induced GroEL expression of Oscillatoria sp. NKBG 091600. Furthermore, this UV-A-inducible GroEL may function to upregulate biopterin glucoside biosynthesis, thereby allowing growth under UV-A irradiation.
| Original language | English |
|---|---|
| Pages (from-to) | 949-954 |
| Number of pages | 6 |
| Journal | Microbiology |
| Volume | 145 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 Apr |
| Externally published | Yes |
Keywords
- Biopterin glucoside
- GroEL
- Marine cyanobacterium Oscillatoria sp.
- Stress reponse
- UV-A resistance
ASJC Scopus subject areas
- Microbiology
