UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels

Bjorn Renberg, Kazuma Mawatari, Takehiko Tsukahara, Naokazu Idota, Kae Sato, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages1528-1530
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CitySan Diego, CA
Period08/10/1208/10/16

Fingerprint

Ligands
Masks
Fused silica
DNA
Fabrication
Glass

Keywords

  • DNA
  • Extended nanospace
  • Ligand immobilization
  • Nanochannel
  • UV

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Renberg, B., Mawatari, K., Tsukahara, T., Idota, N., Sato, K., & Kitamori, T. (2008). UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1528-1530). Chemical and Biological Microsystems Society.

UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. / Renberg, Bjorn; Mawatari, Kazuma; Tsukahara, Takehiko; Idota, Naokazu; Sato, Kae; Kitamori, Takehiko.

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1528-1530.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Renberg, B, Mawatari, K, Tsukahara, T, Idota, N, Sato, K & Kitamori, T 2008, UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1528-1530, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
Renberg B, Mawatari K, Tsukahara T, Idota N, Sato K, Kitamori T. UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 1528-1530
Renberg, Bjorn ; Mawatari, Kazuma ; Tsukahara, Takehiko ; Idota, Naokazu ; Sato, Kae ; Kitamori, Takehiko. / UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 1528-1530
@inproceedings{32482e9dc6c5463a8360483c9fb78cf4,
title = "UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels",
abstract = "A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.",
keywords = "DNA, Extended nanospace, Ligand immobilization, Nanochannel, UV",
author = "Bjorn Renberg and Kazuma Mawatari and Takehiko Tsukahara and Naokazu Idota and Kae Sato and Takehiko Kitamori",
year = "2008",
language = "English",
pages = "1528--1530",
booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels

AU - Renberg, Bjorn

AU - Mawatari, Kazuma

AU - Tsukahara, Takehiko

AU - Idota, Naokazu

AU - Sato, Kae

AU - Kitamori, Takehiko

PY - 2008

Y1 - 2008

N2 - A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.

AB - A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.

KW - DNA

KW - Extended nanospace

KW - Ligand immobilization

KW - Nanochannel

KW - UV

UR - http://www.scopus.com/inward/record.url?scp=84902438925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902438925&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84902438925

SP - 1528

EP - 1530

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

PB - Chemical and Biological Microsystems Society

ER -