Abstract
A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.
| Original language | English |
|---|---|
| Title of host publication | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1528-1530 |
| Number of pages | 3 |
| Publication status | Published - 2008 |
| Externally published | Yes |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA Duration: 2008 Oct 12 → 2008 Oct 16 |
Other
| Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| City | San Diego, CA |
| Period | 08/10/12 → 08/10/16 |
Keywords
- DNA
- Extended nanospace
- Ligand immobilization
- Nanochannel
- UV
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering