UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels

Bjorn Renberg, Kazuma Mawatari, Takehiko Tsukahara, Naokazu Idota, Kae Sato, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages1528-1530
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CitySan Diego, CA
Period08/10/1208/10/16

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Keywords

  • DNA
  • Extended nanospace
  • Ligand immobilization
  • Nanochannel
  • UV

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Renberg, B., Mawatari, K., Tsukahara, T., Idota, N., Sato, K., & Kitamori, T. (2008). UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1528-1530). Chemical and Biological Microsystems Society.