V-ATPase-dependent luminal acidification is required for endocytic recycling of a yeast cell wall stress sensor, Wsc1p

Kazuma Ueno, Mayu Saito, Makiko Nagashima, Ai Kojima, Show Nishinoaki, Junko Y. Toshima, Jiro Toshima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Wsc1p is a major cell wall sensor protein localized at the polarized cell surface. The localization of Wsc1p is maintained by endocytosis and recycling from endosomes back to the cell surface, but changes to the vacuole when cells are subjected to heat stress. Exploiting this unique property of Wsc1p, we screened for yeast single-gene deletion mutants exhibiting defects in Wsc1p trafficking. By expressing 3GFP-tagged Wsc1p in mutants with deleted genes whose function is related to intracellular trafficking, we identified 5 gene groups affecting Wsc1p trafficking, impaired respectively in endocytic internalization, multivesicular body sorting, the GARP complex, endosomal maturation/vacuolar fusion, and V-ATPase. Interestingly, deletion of the VPH1 gene, encoding the Vo subunit of vacuolar-type H+-ATPase (V-ATPase), led to mis-localization of Wsc1p from the plasma membrane to the vacuole. In addition, disruption of other V-ATPase subunits (vma mutants) also caused defects of Wsc1p trafficking and vacuolar acidification similar to those seen in the vph1Δ mutant. Moreover, we found that deletion of the VPS26 gene, encoding a subunit of the retromer complex, also caused a defect in Wsc1p recycling and mis-localization of Wsc1p to the vacuole. These findings clarified the previously unidentified Wsc1p recycling pathway and requirement of V-ATPase-dependent luminal acidification for Wsc1p recycling.

Original languageEnglish
Pages (from-to)549-555
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume443
Issue number2
DOIs
Publication statusPublished - 2014 Jan 10

Fingerprint

Vacuolar Proton-Translocating ATPases
Acidification
Yeast
Cell Wall
Recycling
Gene Deletion
Vacuoles
Yeasts
Cells
Gene encoding
Genes
Sensors
Defects
Multivesicular Bodies
Endosomes
Cell membranes
Endocytosis
Sorting
Fusion reactions
Hot Temperature

Keywords

  • Endocytosis
  • Recycling
  • Retromer complex
  • V-ATPase
  • Wsc1p

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

V-ATPase-dependent luminal acidification is required for endocytic recycling of a yeast cell wall stress sensor, Wsc1p. / Ueno, Kazuma; Saito, Mayu; Nagashima, Makiko; Kojima, Ai; Nishinoaki, Show; Toshima, Junko Y.; Toshima, Jiro.

In: Biochemical and Biophysical Research Communications, Vol. 443, No. 2, 10.01.2014, p. 549-555.

Research output: Contribution to journalArticle

Ueno, Kazuma ; Saito, Mayu ; Nagashima, Makiko ; Kojima, Ai ; Nishinoaki, Show ; Toshima, Junko Y. ; Toshima, Jiro. / V-ATPase-dependent luminal acidification is required for endocytic recycling of a yeast cell wall stress sensor, Wsc1p. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 443, No. 2. pp. 549-555.
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