Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Maki Ohtani, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aoki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.

Original languageEnglish
Pages (from-to)724-728
Number of pages5
JournalFish and Shellfish Immunology
Volume34
Issue number2
DOIs
Publication statusPublished - 2013 Feb

Keywords

  • IgNAR
  • Neutralization activity
  • Phage display library
  • VHSV

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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