Variation and estimation of the differential absorption coefficient of P-700 in spinach photosystem I preparations

Kintake Sonoike, Sakae Katoh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Treatment of spinach Photosystem I particles with detergents induced an apparent blue shift of chlorophyll α at 690 nm in the difference spectrum of P-700. As a consequence of the band shift, the differential absorption coefficient of P-700 was increased from 63 to 87 mM-1 .cm-1. A curve was presented, with which changes in the apparent differential absorption coefficient of P-700 can be estimated by measuring magnitudes of light-induced absorption decreases at 680 nm and 700 nm. The curve was compared with that for cyanobacterial preparations and the mechanism of the detergent-induced band shift was discussed.

Original languageEnglish
Pages (from-to)1079-1082
Number of pages4
JournalPlant and Cell Physiology
Volume31
Issue number8
Publication statusPublished - 1990 Dec 1

Keywords

  • Detergent
  • Differential absorption coefficient
  • Electrochromic shift
  • P-700
  • Photosystem
  • Spinach

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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