Vasoactive intestinal peptide produces long-lasting changes in neural activity in the suprachiasmatic nucleus

Takashi Kudo, Yu Tahara, Karen L. Gamble, Douglas G. McMahon, Gene D. Block, Christopher S. Colwell

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The neuropeptide vasoactive intestinal peptide (VIP) is expressed at high levels in the neurons of the suprachiasmatic nucleus (SCN). While VIP is known to be important to the input and output pathways from the SCN, the physiological effects of VIP on electrical activity of SCN neurons are not well known. Here the impact of VIP on firing rate of SCN neurons was investigated in mouse slice cultures recorded during the night. The application of VIP produced an increase in electrical activity in SCN slices that lasted several hours after treatment. This is a novel mechanism by which this peptide can produce long-term changes in central nervous system physiology. The increase in action potential frequency was blocked by a VIP receptor antagonist and lost in a VIP receptor knockout mouse. In addition, inhibitors of both the Epac family of cAMP binding proteins and cAMP-dependent protein kinase (PKA) blocked the induction by VIP. The persistent increase in spike rate following VIP application was not seen in SCN neurons from mice deficient in Kv3 channel proteins and was dependent on the clock protein PER1. These findings suggest that VIP regulates the long-term firing rate of SCN neurons through a VIPR2-mediated increase in the cAMP pathway and implicate the fast delayed rectifier (FDR) potassium currents as one of the targets of this regulation.

Original languageEnglish
Pages (from-to)1097-1106
Number of pages10
JournalJournal of Neurophysiology
Volume110
Issue number5
DOIs
Publication statusPublished - 2013 Sep 1
Externally publishedYes

Fingerprint

Suprachiasmatic Nucleus
Vasoactive Intestinal Peptide
Neurons
Vasoactive Intestinal Peptide Receptors
Nervous System Physiological Phenomena
Cyclic AMP-Dependent Protein Kinases
Neuropeptides
Knockout Mice
Action Potentials
Potassium
Carrier Proteins
Proteins
Central Nervous System
Peptides

Keywords

  • Circadian system
  • Fast delayed rectifier
  • Potassium
  • Suprachiasmatic nucleus
  • VIP

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Vasoactive intestinal peptide produces long-lasting changes in neural activity in the suprachiasmatic nucleus. / Kudo, Takashi; Tahara, Yu; Gamble, Karen L.; McMahon, Douglas G.; Block, Gene D.; Colwell, Christopher S.

In: Journal of Neurophysiology, Vol. 110, No. 5, 01.09.2013, p. 1097-1106.

Research output: Contribution to journalArticle

Kudo, Takashi ; Tahara, Yu ; Gamble, Karen L. ; McMahon, Douglas G. ; Block, Gene D. ; Colwell, Christopher S. / Vasoactive intestinal peptide produces long-lasting changes in neural activity in the suprachiasmatic nucleus. In: Journal of Neurophysiology. 2013 ; Vol. 110, No. 5. pp. 1097-1106.
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