Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed

Mami Tanaka, Shoko Endo, Fumihito Kotake, Nurhidayu Al-Saari, A. K.M.Rohul Amin, Gao Feng, Sayaka Mino, Hidetaka Doi, Yoshitoshi Ogura, Tetsuya Hayashi, Wataru Suda, Masahira Hattori, Isao Yumoto, Toko Sawabe, Tomoo Sawabe, Toshiyoshi Araki

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were nonmotile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).

    Original languageEnglish
    Article numbere0180053
    JournalPLoS One
    Volume12
    Issue number6
    DOIs
    Publication statusPublished - 2017 Jun 1

    Fingerprint

    Seaweed
    Multilocus Sequence Typing
    Vibrio
    rRNA Genes
    macroalgae
    Genome
    new species
    DNA
    Base Composition
    Amylases
    Genes
    Lipase
    Names
    Carbon
    Gases
    Temperature
    Growth
    Vibrio rumoiensis
    Proteins
    ribosomal RNA

    ASJC Scopus subject areas

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Tanaka, M., Endo, S., Kotake, F., Al-Saari, N., Amin, A. K. M. R., Feng, G., ... Araki, T. (2017). Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. PLoS One, 12(6), [e0180053]. https://doi.org/10.1371/journal.pone.0180053

    Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. / Tanaka, Mami; Endo, Shoko; Kotake, Fumihito; Al-Saari, Nurhidayu; Amin, A. K.M.Rohul; Feng, Gao; Mino, Sayaka; Doi, Hidetaka; Ogura, Yoshitoshi; Hayashi, Tetsuya; Suda, Wataru; Hattori, Masahira; Yumoto, Isao; Sawabe, Toko; Sawabe, Tomoo; Araki, Toshiyoshi.

    In: PLoS One, Vol. 12, No. 6, e0180053, 01.06.2017.

    Research output: Contribution to journalArticle

    Tanaka, M, Endo, S, Kotake, F, Al-Saari, N, Amin, AKMR, Feng, G, Mino, S, Doi, H, Ogura, Y, Hayashi, T, Suda, W, Hattori, M, Yumoto, I, Sawabe, T, Sawabe, T & Araki, T 2017, 'Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed' PLoS One, vol. 12, no. 6, e0180053. https://doi.org/10.1371/journal.pone.0180053
    Tanaka M, Endo S, Kotake F, Al-Saari N, Amin AKMR, Feng G et al. Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. PLoS One. 2017 Jun 1;12(6). e0180053. https://doi.org/10.1371/journal.pone.0180053
    Tanaka, Mami ; Endo, Shoko ; Kotake, Fumihito ; Al-Saari, Nurhidayu ; Amin, A. K.M.Rohul ; Feng, Gao ; Mino, Sayaka ; Doi, Hidetaka ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Suda, Wataru ; Hattori, Masahira ; Yumoto, Isao ; Sawabe, Toko ; Sawabe, Tomoo ; Araki, Toshiyoshi. / Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. In: PLoS One. 2017 ; Vol. 12, No. 6.
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    abstract = "A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1{\%} based on the genome sequence. Major traits of the strain were nonmotile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).",
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    AU - Amin, A. K.M.Rohul

    AU - Feng, Gao

    AU - Mino, Sayaka

    AU - Doi, Hidetaka

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    AU - Hayashi, Tetsuya

    AU - Suda, Wataru

    AU - Hattori, Masahira

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