Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan

Feng Gao, Nurhidayu Al-saari, A. K M Rohul Amin, Kazumichi Sato, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Moriya Ohkuma, Paulo Iiboshi Hargreaves, Pedro Milet Meirelles, Fabiano L. Thompson, Cristiane Thompson, Bruno Gomez-Gil, Toko Sawabe, Tomoo Sawabe

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off Okinawa in coral reef areas. These strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (MLSA) and in silico DNA-DNA similarity (in silico DDH). Phylogenetic analyses on the basis of 16S rRNA gene sequences revealed that the isolates could be assigned to the genus Vibrio, however they were not allocated into any distinct cluster with known Vibrionaceae species. MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the vibrios formed an outskirt branch of Halioticoli clade. The experimental DNA-DNA hybridization data revealed that the five strains were in the range of being defined as conspecific but separate from nine Halioticoli clade species. The G+C contents of the Vibrio ishigakensis strains were 47.3-49.1mol%. Both Amino Acid Identity and Average Nucleotide Identity of the strain C1T against Vibrio ezurae HDS1-1T, Vibrio gallicus HT2-1T, Vibrio halioticoli IAM 14596T, Vibrio neonatus HDD3-1T and Vibrio superstes G3-29T showed less than 95% similarity. The genome-based taxonomic approach by means of in silico DDH values also supports the V. ishigakensis strains being distinct from the other known Halioticoli clade species. Sixteen traits (growth temperature range, DNase and lipase production, indole production, and assimilation of 10 carbon compounds) distinguished these strains from Halioticoli clade species. The names V. ishigakensis sp. nov. is proposed for the species of Halioticoli clade, with C1T as the type strain (JCM 19231T =LMG 28703T).

    Original languageEnglish
    JournalSystematic and Applied Microbiology
    DOIs
    Publication statusAccepted/In press - 2015 Aug 30

    Fingerprint

    Coral Reefs
    Vibrio
    Ryukyu Archipelago
    Seawater
    coral reefs
    coral reef
    Japan
    seawater
    new species
    DNA
    Computer Simulation
    Multilocus Sequence Typing
    genome
    gene
    Vibrio halioticoli
    Vibrionaceae
    Genome
    taxonomy
    deoxyribonucleases
    amino acid

    Keywords

    • Coral reef
    • Genome taxonomy
    • Halioticoli clade
    • Seawater
    • Vibrio
    • Vibrionaceae

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics
    • Applied Microbiology and Biotechnology

    Cite this

    Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan. / Gao, Feng; Al-saari, Nurhidayu; Rohul Amin, A. K M; Sato, Kazumichi; Mino, Sayaka; Suda, Wataru; Oshima, Kenshiro; Hattori, Masahira; Ohkuma, Moriya; Hargreaves, Paulo Iiboshi; Meirelles, Pedro Milet; Thompson, Fabiano L.; Thompson, Cristiane; Gomez-Gil, Bruno; Sawabe, Toko; Sawabe, Tomoo.

    In: Systematic and Applied Microbiology, 30.08.2015.

    Research output: Contribution to journalArticle

    Gao, F, Al-saari, N, Rohul Amin, AKM, Sato, K, Mino, S, Suda, W, Oshima, K, Hattori, M, Ohkuma, M, Hargreaves, PI, Meirelles, PM, Thompson, FL, Thompson, C, Gomez-Gil, B, Sawabe, T & Sawabe, T 2015, 'Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan', Systematic and Applied Microbiology. https://doi.org/10.1016/j.syapm.2016.04.002
    Gao, Feng ; Al-saari, Nurhidayu ; Rohul Amin, A. K M ; Sato, Kazumichi ; Mino, Sayaka ; Suda, Wataru ; Oshima, Kenshiro ; Hattori, Masahira ; Ohkuma, Moriya ; Hargreaves, Paulo Iiboshi ; Meirelles, Pedro Milet ; Thompson, Fabiano L. ; Thompson, Cristiane ; Gomez-Gil, Bruno ; Sawabe, Toko ; Sawabe, Tomoo. / Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan. In: Systematic and Applied Microbiology. 2015.
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    AU - Al-saari, Nurhidayu

    AU - Rohul Amin, A. K M

    AU - Sato, Kazumichi

    AU - Mino, Sayaka

    AU - Suda, Wataru

    AU - Oshima, Kenshiro

    AU - Hattori, Masahira

    AU - Ohkuma, Moriya

    AU - Hargreaves, Paulo Iiboshi

    AU - Meirelles, Pedro Milet

    AU - Thompson, Fabiano L.

    AU - Thompson, Cristiane

    AU - Gomez-Gil, Bruno

    AU - Sawabe, Toko

    AU - Sawabe, Tomoo

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    N2 - Five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off Okinawa in coral reef areas. These strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (MLSA) and in silico DNA-DNA similarity (in silico DDH). Phylogenetic analyses on the basis of 16S rRNA gene sequences revealed that the isolates could be assigned to the genus Vibrio, however they were not allocated into any distinct cluster with known Vibrionaceae species. MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the vibrios formed an outskirt branch of Halioticoli clade. The experimental DNA-DNA hybridization data revealed that the five strains were in the range of being defined as conspecific but separate from nine Halioticoli clade species. The G+C contents of the Vibrio ishigakensis strains were 47.3-49.1mol%. Both Amino Acid Identity and Average Nucleotide Identity of the strain C1T against Vibrio ezurae HDS1-1T, Vibrio gallicus HT2-1T, Vibrio halioticoli IAM 14596T, Vibrio neonatus HDD3-1T and Vibrio superstes G3-29T showed less than 95% similarity. The genome-based taxonomic approach by means of in silico DDH values also supports the V. ishigakensis strains being distinct from the other known Halioticoli clade species. Sixteen traits (growth temperature range, DNase and lipase production, indole production, and assimilation of 10 carbon compounds) distinguished these strains from Halioticoli clade species. The names V. ishigakensis sp. nov. is proposed for the species of Halioticoli clade, with C1T as the type strain (JCM 19231T =LMG 28703T).

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