Virus inactivation in hemoglobin solution by heat treatment

H. Abe, K. Ikebuchi, J. Hirayama, M. Fujihara, Shinji Takeoka, H. Sakai, E. Tsuchida, H. Ikeda

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60°C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.8 log10 and >6.0 log 10under the air and CO atmosphere, respectively. Although the methemoglobin rate increased after the heat treatment under the air atmosphere, no methemoglobin formation was observed by the treatment under the CO atmosphere. Isoelectric focusing analysis revealed the denaturation of hemoglobin after the heat treatment under the air, while hemoglobin banding was not altered in the carbonylated condition. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both con ditions. In addition, the hemoglobin concentration in the SFH was higher after the heat treatment than before the treatment. These findings indicate that the heat treatment under the CO atmosphere inactivates viruses without hemoglobin denaturation, and hence, this method is a promising approach to prepare a safer SFH as artificial oxygen carriers.

    Original languageEnglish
    Pages (from-to)381-388
    Number of pages8
    JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
    Volume29
    Issue number5
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Virus Inactivation
    Hemoglobin
    Viruses
    Hemoglobins
    Hot Temperature
    Heat treatment
    Atmosphere
    Carbon Monoxide
    Air
    Methemoglobin
    Vesicular Stomatitis
    Denaturation
    Oxygen
    Isoelectric Focusing
    Polyacrylamide Gel Electrophoresis
    Safety

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Hematology
    • Biotechnology
    • Biomaterials

    Cite this

    Virus inactivation in hemoglobin solution by heat treatment. / Abe, H.; Ikebuchi, K.; Hirayama, J.; Fujihara, M.; Takeoka, Shinji; Sakai, H.; Tsuchida, E.; Ikeda, H.

    In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 29, No. 5, 2001, p. 381-388.

    Research output: Contribution to journalArticle

    Abe, H, Ikebuchi, K, Hirayama, J, Fujihara, M, Takeoka, S, Sakai, H, Tsuchida, E & Ikeda, H 2001, 'Virus inactivation in hemoglobin solution by heat treatment', Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, vol. 29, no. 5, pp. 381-388. https://doi.org/10.1081/BIO-100106921
    Abe, H. ; Ikebuchi, K. ; Hirayama, J. ; Fujihara, M. ; Takeoka, Shinji ; Sakai, H. ; Tsuchida, E. ; Ikeda, H. / Virus inactivation in hemoglobin solution by heat treatment. In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology. 2001 ; Vol. 29, No. 5. pp. 381-388.
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