Vision system for animal cell recognition in a bio-engineering process

Toshio Fukuda, Mitsuru Ishizuka, Osamu Hasegawa, Hajime Asama, Teruyuki Nagamune, Isao Endo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An expert system for the recognition and counting of cells using image processing for mass production of interferon beta is described. The biological cells which make interferon beta are taken from animals and cultured on microcarriers, plastic balls/beads 150-200 μm in diameter floating in a culture solution. Cell images are obtained through a microscope connected to a TV camera. The number of cells in the culture vessel increases during a six-day period, and the expert system recognizes the state of cell growth during this time by means of image processing. The expert system is a more effective way to recognize and cluster cells on densely grown/distributed beads from the gray image data than simple image processing. The creature image segmentation method was also applied, and it is shown that this method can recognize the cell shape and location, avoiding individual differences on the plastic balls.

Original languageEnglish
Title of host publicationIECON Proceedings (Industrial Electronics Conference)
Place of PublicationLos Alamitos, CA, United States
PublisherPubl by IEEE
Pages552-557
Number of pages6
Volume1
ISBN (Print)0879426004
Publication statusPublished - 1990
Externally publishedYes
Event16th Annual Conference of IEEE Industrial Electronics Society - IECON'90 - Pacific Grove, CA, USA
Duration: 1990 Nov 271990 Nov 30

Other

Other16th Annual Conference of IEEE Industrial Electronics Society - IECON'90
CityPacific Grove, CA, USA
Period90/11/2790/11/30

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Fukuda, T., Ishizuka, M., Hasegawa, O., Asama, H., Nagamune, T., & Endo, I. (1990). Vision system for animal cell recognition in a bio-engineering process. In IECON Proceedings (Industrial Electronics Conference) (Vol. 1, pp. 552-557). Publ by IEEE.