Visualization of liposomes carrying fibrinogen γ-chain dodecapeptide accumulated to sites of vascular injury using computed tomography

Yosuke Okamura, Kaoruko Eto, Hitomi Maruyama, Makoto Handa, Yasuo Ikeda, Shinji Takeoka

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    We have constructed liposomes with hemostatic activity as a platelet substitute using moderately thrombocytopenic rats. The liposomes were conjugated with the dodecapeptide (HHLGGAKQAGDV: H12), which is a fibrinogen γ-chain C-terminal sequence (γ 400-411). To visualize liposome accumulation at the site of vascular injury by in vivo computed tomography, a water-soluble contrast dye, N,N′-bis[2-hydroxy-1-(hydroxylmethyl)ethyl]-5-[(2S)-2-hydroxylprop anoylamino]-2,4,6-triiodoisophthalamide (iopamidol), was encapsulated into the H12-conjugated liposomes. We achieved direct visualization of specific accumulation of the H12-(iopamidol)liposomes at the jugular vein injured by ferric chloride and succeeded in semiquantitative analyses of the accumulated amount of H12-liposomes in the injured site. We therefore propose that H12-liposomes that are specifically recruited to, and exert their hemostatic activity at the site of vascular injury, have a significant potential as a carrier and/or as an ideal platelet substitute. Furthermore, the H12-(iopamidol)liposomes would also be clinically useful as diagnostic agents for pathological thrombus detection and as contrast dyes for hepatosplenography. From the Clinical Editor: The authors have constructed liposomes with hemostatic activity as a platelet substitute using moderately thrombocytopenic rats. They propose that H12-liposomes that are specifically recruited to, and exert their hemostatic activity at the site of vascular injury, have a significant potential as a carrier and/or as an ideal platelet substitute. Furthermore, the H12-(iopamidol) liposomes would also be clinically useful as diagnostic agents for thrombus detection and as contrast dyes for hepatosplenography.

    Original languageEnglish
    Pages (from-to)391-396
    Number of pages6
    JournalNanomedicine: Nanotechnology, Biology, and Medicine
    Volume6
    Issue number2
    DOIs
    Publication statusPublished - 2010 Apr

    Fingerprint

    Liposomes
    Vascular System Injuries
    Fibrinogen
    Tomography
    Visualization
    Iopamidol
    Hemostatics
    Platelets
    Blood Platelets
    Coloring Agents
    Dyes
    Rats
    Thrombosis
    Jugular Veins

    Keywords

    • Computed tomography
    • Dodecapeptide
    • Iopamidol
    • Liposome
    • Platelet substitute

    ASJC Scopus subject areas

    • Molecular Medicine
    • Bioengineering
    • Biomedical Engineering
    • Materials Science(all)
    • Medicine (miscellaneous)
    • Pharmaceutical Science

    Cite this

    Visualization of liposomes carrying fibrinogen γ-chain dodecapeptide accumulated to sites of vascular injury using computed tomography. / Okamura, Yosuke; Eto, Kaoruko; Maruyama, Hitomi; Handa, Makoto; Ikeda, Yasuo; Takeoka, Shinji.

    In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 6, No. 2, 04.2010, p. 391-396.

    Research output: Contribution to journalArticle

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