TY - JOUR
T1 - Visualization of mPer1 transcription in vitro
T2 - NMDA induces a rapid phase shift of mPer1 gene in cultured SCN
AU - Asai, Makoto
AU - Yamaguchi, Shun
AU - Isejima, Hiromi
AU - Jonouchi, Masafumi
AU - Moriya, Takahiro
AU - Shibata, Shigenobu
AU - Kobayashi, Masaki
AU - Okamura, Hitoshi
N1 - Funding Information:
Dr. Keiko Tominaga is acknowledged for the introduction of SCN slice culture. We thank Chikako Koike for assistance with manuscript preparation. This work was supported in part by grants from the Special Coordination Funds of the Science and Technology Agency of Japan; the Grant in Aid for the Scientific Research on Priority Areas of the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2001/10/2
Y1 - 2001/10/2
N2 - Many physiological and behavioral phenomena are controlled by an internal, self-sustaining oscillator with a periodicity of approximately 24 hr. In mammals, the principal oscillator resides in the suprachiasmatic nucleus (SCN). A light pulse during the subjective night causes a phase shift of the circadian rhythm via direct glutamatergic retinal afferents to the SCN [1]. Along with the accepted theoretical models of the clock, it is suggested that behavioral resetting of mammals is completed within 2 hr [2]; however, the molecular mechanism has not been elucidated. Here, we show the real-time image of the transcription of the circadian-clock gene mPer1 in the cultured SCN by using the transgenic mice that carry a luciferase reporter gene under the control of the mPer1 promoter [3]. The real-time image demonstrates that the mPer1 promoter activity oscillates robustly in a circadian manner and that this promoter activity is reset rapidly (within 2-3 hr) when a phase shift occurs.
AB - Many physiological and behavioral phenomena are controlled by an internal, self-sustaining oscillator with a periodicity of approximately 24 hr. In mammals, the principal oscillator resides in the suprachiasmatic nucleus (SCN). A light pulse during the subjective night causes a phase shift of the circadian rhythm via direct glutamatergic retinal afferents to the SCN [1]. Along with the accepted theoretical models of the clock, it is suggested that behavioral resetting of mammals is completed within 2 hr [2]; however, the molecular mechanism has not been elucidated. Here, we show the real-time image of the transcription of the circadian-clock gene mPer1 in the cultured SCN by using the transgenic mice that carry a luciferase reporter gene under the control of the mPer1 promoter [3]. The real-time image demonstrates that the mPer1 promoter activity oscillates robustly in a circadian manner and that this promoter activity is reset rapidly (within 2-3 hr) when a phase shift occurs.
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U2 - 10.1016/S0960-9822(01)00445-6
DO - 10.1016/S0960-9822(01)00445-6
M3 - Article
C2 - 11591320
AN - SCOPUS:0035797924
SN - 0960-9822
VL - 11
SP - 1524
EP - 1527
JO - Current Biology
JF - Current Biology
IS - 19
ER -