Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo; Toru Sasaki; Yasunori Sato; Akiko Amano; Shingo Aizawa; Mizuki Iwama; Setsuko Handa; Nobuko Shimada; Mitsugu Fukuda; Masumi Akita; Jaewon Lee; Kyu Shik Jeong; Naoki Maruyama; Akihito Ishigami

doi:10.1016/j.bbrc.2008.09.132

Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo, Toru Sasaki, Yasunori Sato, Akiko Amano, Shingo Aizawa, Mizuki Iwama, Setsuko Handa, Nobuko Shimada, Mitsugu Fukuda, Masumi Akita, Jaewon Lee, Kyu Shik Jeong, Naoki Maruyama, Akihito Ishigami

School of Human Sciences

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

Original language	English
Pages (from-to)	291-296
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	377
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.09.132
Publication status	Published - 2008 Dec 5

Keywords

Ascorbic acid
Catalase
Chemiluminescence
Gluconolactonase
Oxidative stress
ROS
SMP30
SOD
Senescence marker protein-30
Vitamin C

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kondo, Y., Sasaki, T., Sato, Y., Amano, A., Aizawa, S., Iwama, M., Handa, S., Shimada, N., Fukuda, M., Akita, M., Lee, J., Jeong, K. S., Maruyama, N., & Ishigami, A. (2008). Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. Biochemical and Biophysical Research Communications, 377(1), 291-296. https://doi.org/10.1016/j.bbrc.2008.09.132

Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. / Kondo, Yoshitaka; Sasaki, Toru; Sato, Yasunori; Amano, Akiko; Aizawa, Shingo; Iwama, Mizuki; Handa, Setsuko; Shimada, Nobuko; Fukuda, Mitsugu; Akita, Masumi; Lee, Jaewon; Jeong, Kyu Shik; Maruyama, Naoki; Ishigami, Akihito.

In: Biochemical and Biophysical Research Communications, Vol. 377, No. 1, 05.12.2008, p. 291-296.

Research output: Contribution to journal › Article › peer-review

Kondo, Y, Sasaki, T, Sato, Y, Amano, A, Aizawa, S, Iwama, M, Handa, S, Shimada, N, Fukuda, M, Akita, M, Lee, J, Jeong, KS, Maruyama, N & Ishigami, A 2008, 'Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice', Biochemical and Biophysical Research Communications, vol. 377, no. 1, pp. 291-296. https://doi.org/10.1016/j.bbrc.2008.09.132

Kondo, Yoshitaka ; Sasaki, Toru ; Sato, Yasunori ; Amano, Akiko ; Aizawa, Shingo ; Iwama, Mizuki ; Handa, Setsuko ; Shimada, Nobuko ; Fukuda, Mitsugu ; Akita, Masumi ; Lee, Jaewon ; Jeong, Kyu Shik ; Maruyama, Naoki ; Ishigami, Akihito. / Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 377, No. 1. pp. 291-296.

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title = "Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice",

abstract = "Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.",

keywords = "Ascorbic acid, Catalase, Chemiluminescence, Gluconolactonase, Oxidative stress, ROS, SMP30, SOD, Senescence marker protein-30, Vitamin C",

author = "Yoshitaka Kondo and Toru Sasaki and Yasunori Sato and Akiko Amano and Shingo Aizawa and Mizuki Iwama and Setsuko Handa and Nobuko Shimada and Mitsugu Fukuda and Masumi Akita and Jaewon Lee and Jeong, {Kyu Shik} and Naoki Maruyama and Akihito Ishigami",

note = "Funding Information: This study is supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture, Japan (to A.I., S.H. and N.S.), an award from Health Science Research Grants for Comprehensive Research on Aging and Health supported by the Ministry of Health, Labor, and Welfare, Japan (to N.M.), and Grant-in-Aid from the Asahi Breweries Foundation, Japan (to A.I.) and Smoking Research Foundation, Japan (to A.I.). We thank Ms. P. Minick for the excellent English editorial assistance. Vitamin C powder was kindly provided by DSM Nutrition Japan.",

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AU - Kondo, Yoshitaka

AU - Sasaki, Toru

AU - Sato, Yasunori

AU - Amano, Akiko

AU - Aizawa, Shingo

AU - Iwama, Mizuki

AU - Handa, Setsuko

AU - Shimada, Nobuko

AU - Fukuda, Mitsugu

AU - Akita, Masumi

AU - Lee, Jaewon

AU - Jeong, Kyu Shik

AU - Maruyama, Naoki

AU - Ishigami, Akihito

N1 - Funding Information: This study is supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture, Japan (to A.I., S.H. and N.S.), an award from Health Science Research Grants for Comprehensive Research on Aging and Health supported by the Ministry of Health, Labor, and Welfare, Japan (to N.M.), and Grant-in-Aid from the Asahi Breweries Foundation, Japan (to A.I.) and Smoking Research Foundation, Japan (to A.I.). We thank Ms. P. Minick for the excellent English editorial assistance. Vitamin C powder was kindly provided by DSM Nutrition Japan.

PY - 2008/12/5

Y1 - 2008/12/5

N2 - Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

AB - Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

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KW - Senescence marker protein-30

KW - Vitamin C

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