Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo, Toru Sasaki, Yasunori Sato, Akiko Amano, Shingo Aizawa, Mizuki Iwama, Setsuko Handa, Nobuko Shimada, Mitsugu Fukuda, Masumi Akita, Jaewon Lee, Kyu Shik Jeong, Naoki Maruyama, Akihito Ishigami

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number1
DOIs
Publication statusPublished - 2008 Dec 5
Externally publishedYes

Keywords

  • Ascorbic acid
  • Catalase
  • Chemiluminescence
  • Gluconolactonase
  • Oxidative stress
  • ROS
  • Senescence marker protein-30
  • SMP30
  • SOD
  • Vitamin C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kondo, Y., Sasaki, T., Sato, Y., Amano, A., Aizawa, S., Iwama, M., Handa, S., Shimada, N., Fukuda, M., Akita, M., Lee, J., Jeong, K. S., Maruyama, N., & Ishigami, A. (2008). Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. Biochemical and Biophysical Research Communications, 377(1), 291-296. https://doi.org/10.1016/j.bbrc.2008.09.132