Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo; Toru Sasaki; Yasunori Sato; Akiko Amano; Shingo Aizawa; Mizuki Iwama; Setsuko Handa; Nobuko Shimada; Mitsugu Fukuda; Masumi Akita; Jaewon Lee; Kyu Shik Jeong; Naoki Maruyama; Akihito Ishigami

doi:10.1016/j.bbrc.2008.09.132

Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo, Toru Sasaki, Yasunori Sato, Akiko Amano, Shingo Aizawa, Mizuki Iwama, Setsuko Handa, Nobuko Shimada, Mitsugu Fukuda, Masumi Akita, Jaewon Lee, Kyu Shik Jeong, Naoki Maruyama, Akihito Ishigami

Research output: Contribution to journal › Article

64 Citations (Scopus)

Abstract

Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

Original language	English
Pages (from-to)	291-296
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	377
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.09.132
Publication status	Published - 2008 Dec 5
Externally published	Yes

Keywords

Ascorbic acid
Catalase
Chemiluminescence
Gluconolactonase
Oxidative stress
ROS
Senescence marker protein-30
SMP30
SOD
Vitamin C

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kondo, Y., Sasaki, T., Sato, Y., Amano, A., Aizawa, S., Iwama, M., Handa, S., Shimada, N., Fukuda, M., Akita, M., Lee, J., Jeong, K. S., Maruyama, N., & Ishigami, A. (2008). Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. Biochemical and Biophysical Research Communications, 377(1), 291-296. https://doi.org/10.1016/j.bbrc.2008.09.132

Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. / Kondo, Yoshitaka; Sasaki, Toru; Sato, Yasunori; Amano, Akiko; Aizawa, Shingo; Iwama, Mizuki; Handa, Setsuko; Shimada, Nobuko; Fukuda, Mitsugu; Akita, Masumi; Lee, Jaewon; Jeong, Kyu Shik; Maruyama, Naoki; Ishigami, Akihito.

In: Biochemical and Biophysical Research Communications, Vol. 377, No. 1, 05.12.2008, p. 291-296.

Research output: Contribution to journal › Article

Kondo, Y, Sasaki, T, Sato, Y, Amano, A, Aizawa, S, Iwama, M, Handa, S, Shimada, N, Fukuda, M, Akita, M, Lee, J, Jeong, KS, Maruyama, N & Ishigami, A 2008, 'Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice', Biochemical and Biophysical Research Communications, vol. 377, no. 1, pp. 291-296. https://doi.org/10.1016/j.bbrc.2008.09.132

Kondo, Yoshitaka ; Sasaki, Toru ; Sato, Yasunori ; Amano, Akiko ; Aizawa, Shingo ; Iwama, Mizuki ; Handa, Setsuko ; Shimada, Nobuko ; Fukuda, Mitsugu ; Akita, Masumi ; Lee, Jaewon ; Jeong, Kyu Shik ; Maruyama, Naoki ; Ishigami, Akihito. / Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 377, No. 1. pp. 291-296.

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abstract = "Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.",

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