Vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice

Daisuke Hayashi, Hotaka Sugaya, Takayuki Ohkoshi, Kaori Sekizawa, Hirokatsu Takatsu, Tadashi Shinkai, Shiro Urano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We aimed to define whether vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice. After picryl chloride (PC) application to their backs, changes in the content of thiobarbituric acid reactive substances (TBARS) and vitamin E, as well as the activity of antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase) were analyzed in the serum and skin of NC/Nga mice during a symptomatic cycle. The levels of inflammatory factors were also assessed, including IgE, cyclooxigenase-2 (COX-2), tumor necrosis factor (TNF-α) and nuclear factor-κB (NF-κB). When allergic dermatitis was induced by the application of PC to the skin of the mice, skin inflammation appeared 2 wk after PC application, with the peak severity of inflammation observed 5 wk after PC application. Subsequently, the animals recovered from the inflammation by 9 wk after PC application. The TBARS content in the skin and serum increased markedly when the symptoms were the most severe, and decreased to levels near those in control mice by 9 wk after PC application. The activities of SOD and GSHPx in the skin and serum were also positively correlated with symptomatic changes; however, no change in catalase activity was observed 5 wk after PC application. Conversely, vitamin E content decreased at the stage of peak severity. The levels of all inflammatory factors analyzed in this study were altered in a manner similar to other indices. Additionally, vitamin E treatment markedly inhibited these PC-induced alterations. On the basis of these results, it is expected that the observed alterations in biochemical indices, which reflect the symptomatic cycle, may be applicable to objective diagnosis and treatment for atopic dermatitis, and that vitamin E may improve the symptoms of AD.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume58
Issue number3
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Picryl Chloride
Atopic Dermatitis
Vitamin E
Inflammation
Skin
Thiobarbituric Acid Reactive Substances
Catalase
Superoxide Dismutase
Serum
Dermatitis
Glutathione Peroxidase
Immunoglobulin E
Tumor Necrosis Factor-alpha
Antioxidants

Keywords

  • Atopic dermatitis
  • Biochemical indices
  • Cytokine
  • Oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice. / Hayashi, Daisuke; Sugaya, Hotaka; Ohkoshi, Takayuki; Sekizawa, Kaori; Takatsu, Hirokatsu; Shinkai, Tadashi; Urano, Shiro.

In: Journal of Nutritional Science and Vitaminology, Vol. 58, No. 3, 2012, p. 161-168.

Research output: Contribution to journalArticle

Hayashi, Daisuke ; Sugaya, Hotaka ; Ohkoshi, Takayuki ; Sekizawa, Kaori ; Takatsu, Hirokatsu ; Shinkai, Tadashi ; Urano, Shiro. / Vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice. In: Journal of Nutritional Science and Vitaminology. 2012 ; Vol. 58, No. 3. pp. 161-168.
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