Voltage-induced conductance in human erythrocyte membranes

Kazuhiko Kinosita, Tian Yow Tsong

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Isotonic suspensions of erythrocytes were exposed to intense electric fields for a duration in microseconds. Time-dependent increase in the conductivity of the suspension was observed under fields greater than a threshold of about 1.5 kV/cm. The threshold was independent of the ionic strength of the medium, and changed little with temperature or with the rise time of the applied field. Under fields greater than 3 kV/cm, the time course of the conductivity increase consisted of a rapid (approx. 1 μs) and a slow (approx. 100 μs) phases. The increase is attributed primarily to large membrane conductance induced by the applied field. The membrane conductance is in the order of 10 Ω-1/cm2 in the rapid phase and 102 Ω-1/cm2 in the slow phase. Comparison with previous results indicates that this induced membrane conductance corresponds to the formation of aqueous pores in the cell membrane. After the applied field was removed, the conductivity of the suspension returned nearly to its initial value, indicating that the induced membrane conductance is strongly dependent on the membrane potential. The conductivity then increased again in the time range of 10 s. This is attributed to the diffusional efflux of intracellular ions through the voltage-induced pores. From the rate of the efflux, number of the pores/cell is estimated to be in the order of 102. Final stage of the conductivity change was a slow decrease, corresponding to the colloid osmotic swelling of the perforated cells.

Original languageEnglish
Pages (from-to)479-497
Number of pages19
JournalBBA - Biomembranes
Volume554
Issue number2
DOIs
Publication statusPublished - 1979 Jul 5
Externally publishedYes

Fingerprint

Erythrocyte Membrane
Membranes
Suspensions
Electric potential
Colloids
Membrane Potentials
Osmolar Concentration
Cell membranes
Ionic strength
Cell Count
Erythrocytes
Cell Membrane
Swelling
Ions
Temperature
Electric fields

Keywords

  • (Human erythrocyte membrane)
  • Ion permeability
  • Membrane conductance
  • Pore

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Voltage-induced conductance in human erythrocyte membranes. / Kinosita, Kazuhiko; Tsong, Tian Yow.

In: BBA - Biomembranes, Vol. 554, No. 2, 05.07.1979, p. 479-497.

Research output: Contribution to journalArticle

Kinosita, Kazuhiko ; Tsong, Tian Yow. / Voltage-induced conductance in human erythrocyte membranes. In: BBA - Biomembranes. 1979 ; Vol. 554, No. 2. pp. 479-497.
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