Walking nanothermometers: Spatiotemporal temperature measurement of transported acidic organelles in single living cells

Kotaro Oyama, Masao Takabayashi, Yoshiaki Takei, Satoshi Arai, Shinji Takeoka, Shin'Ichi Ishiwata, Madoka Suzuki

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers".

Original languageEnglish
Pages (from-to)1591-1593
Number of pages3
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume12
Issue number9
DOIs
Publication statusPublished - 2012 May 7

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Temperature measurement
Organelles
Walking
Cells
Temperature
Endosomes
Endocytosis
Lysosomes
Ionic strength
HeLa Cells
Microtubules
Nanoparticles
Osmolar Concentration

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Walking nanothermometers : Spatiotemporal temperature measurement of transported acidic organelles in single living cells. / Oyama, Kotaro; Takabayashi, Masao; Takei, Yoshiaki; Arai, Satoshi; Takeoka, Shinji; Ishiwata, Shin'Ichi; Suzuki, Madoka.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 12, No. 9, 07.05.2012, p. 1591-1593.

Research output: Contribution to journalArticle

Oyama, Kotaro ; Takabayashi, Masao ; Takei, Yoshiaki ; Arai, Satoshi ; Takeoka, Shinji ; Ishiwata, Shin'Ichi ; Suzuki, Madoka. / Walking nanothermometers : Spatiotemporal temperature measurement of transported acidic organelles in single living cells. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2012 ; Vol. 12, No. 9. pp. 1591-1593.
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